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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 45, Iss. 6 — Feb. 20, 2006
  • pp: 1225–1235

Spatial fluorescence cross-correlation spectroscopy

Rodolphe Jaffiol, Yoann Blancquaert, Antoine Delon, and Jacques Derouard  »View Author Affiliations

Applied Optics, Vol. 45, Issue 6, pp. 1225-1235 (2006)

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We present an alternative method for diffusion measurements of fluorescent species in solution by use of confocal microscopy and fluorescence correlation spectroscopy techniques. It consists of making a time and spatial dual correlation in which one detects the fluorescence signals from two nearby separate confocal volumes and cross correlates them. To improve the spatial discrimination between the two confocal volumes we propose filtering of fluorescence photocounts by rejecting the fluorescence background, which corresponds to particles located far from the center of the detection volumes.

© 2006 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:

Original Manuscript: July 20, 2005
Manuscript Accepted: September 13, 2005

Virtual Issues
Vol. 1, Iss. 3 Virtual Journal for Biomedical Optics

Rodolphe Jaffiol, Yoann Blancquaert, Antoine Delon, and Jacques Derouard, "Spatial fluorescence cross-correlation spectroscopy," Appl. Opt. 45, 1225-1235 (2006)

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