Spatial fluorescence cross-correlation spectroscopy
Applied Optics, Vol. 45, Issue 6, pp. 1225-1235 (2006)
http://dx.doi.org/10.1364/AO.45.001225
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Abstract
We present an alternative method for diffusion measurements of fluorescent species in solution by use of confocal microscopy and fluorescence correlation spectroscopy techniques. It consists of making a time and spatial dual correlation in which one detects the fluorescence signals from two nearby separate confocal volumes and cross correlates them. To improve the spatial discrimination between the two confocal volumes we propose filtering of fluorescence photocounts by rejecting the fluorescence background, which corresponds to particles located far from the center of the detection volumes.
© 2006 Optical Society of America
OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced
ToC Category:
Spectroscopy
History
Original Manuscript: July 20, 2005
Manuscript Accepted: September 13, 2005
Virtual Issues
Vol. 1, Iss. 3 Virtual Journal for Biomedical Optics
Citation
Rodolphe Jaffiol, Yoann Blancquaert, Antoine Delon, and Jacques Derouard, "Spatial fluorescence cross-correlation spectroscopy," Appl. Opt. 45, 1225-1235 (2006)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-45-6-1225
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