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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 45, Iss. 9 — Mar. 20, 2006
  • pp: 1964–1971

High-resolution angle-resolved measurements of light scattered at small angles by red blood cells in suspension

Ioan Turcu, Cristian V. L. Pop, and Silvia Neamtu  »View Author Affiliations


Applied Optics, Vol. 45, Issue 9, pp. 1964-1971 (2006)
http://dx.doi.org/10.1364/AO.45.001964


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Abstract

Red blood cells (RBCs) scatter light mainly in the forward direction, where the scattering phase function has a narrow peak. We performed an experimental investigation into the angular distribution of light scattered by blood in the small-angle domain. A highly diluted suspension of RBCs (hematocrits in the range 5 × 10 5 10 2 ) was illuminated with a He–Ne laser with 633   nm wavelength. We focused our research on two main topics: the scattering efficiency of the RBCs given by the mean scattering cross section and the scattering anisotropy obtained from the angular distribution of the scattered photons. The collimated beam transmission and the angular distribution of scattered light were measured and compared with the predictions of the effective phase function model. The RBCs' mean scattering cross section and scattering anisotropy were obtained by fitting of the experimental data.

© 2006 Optical Society of America

OCIS Codes
(040.5160) Detectors : Photodetectors
(290.2200) Scattering : Extinction
(290.4210) Scattering : Multiple scattering
(290.5820) Scattering : Scattering measurements
(290.5850) Scattering : Scattering, particles

ToC Category:
Scattering

History
Original Manuscript: July 26, 2005
Revised Manuscript: October 27, 2005
Manuscript Accepted: October 27, 2005

Virtual Issues
Vol. 1, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Ioan Turcu, Cristian V. L. Pop, and Silvia Neamtu, "High-resolution angle-resolved measurements of light scattered at small angles by red blood cells in suspension," Appl. Opt. 45, 1964-1971 (2006)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-45-9-1964


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