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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 45, Iss. 9 — Mar. 20, 2006
  • pp: 2115–2123

Technique for measurement of fluorescence lifetime by use of stroboscopic excitation and continuous-wave detection

D. R. Matthews, H. D. Summers, K. Njoh, R. J. Errington, P. J. Smith, P. Barber, S. Ameer-Beg, and B. Vojnovic  »View Author Affiliations

Applied Optics, Vol. 45, Issue 9, pp. 2115-2123 (2006)

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A study of the practicality a simple technique for obtaining time-domain information that uses continuous wave detection of fluorescence is presented. We show that this technique has potential for use in assays for which a change in the lifetime of an indicator occurs in reaction to an analyte, in fluorescence resonance energy transfer, for example, and could be particularly important when one is carrying out such measurements in the scaled- down environment of a lab on a chip (biochip). A rate-equation model is presented that allows an objective analysis to be made of the relative importance of the key measurement parameters: optical saturation of the fluorophore and period of the excitation pulse. An experimental demonstration of the technique that uses a cuvette-based analysis of a carbocyanine dye and for which the excitation source is a 650   nm wavelength, self-pulsing AlGaInP laser diode is compared with the model.

© 2006 Optical Society of America

OCIS Codes
(140.5960) Lasers and laser optics : Semiconductor lasers
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 8, 2005
Revised Manuscript: October 17, 2005
Manuscript Accepted: October 22, 2005

Virtual Issues
Vol. 1, Iss. 4 Virtual Journal for Biomedical Optics

D. R. Matthews, H. D. Summers, K. Njoh, R. J. Errington, P. J. Smith, P. Barber, S. Ameer-Beg, and B. Vojnovic, "Technique for measurement of fluorescence lifetime by use of stroboscopic excitation and continuous-wave detection," Appl. Opt. 45, 2115-2123 (2006)

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