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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 46, Iss. 10 — Apr. 1, 2007
  • pp: 1650–1657

Sensitivity characterization of a time-domain fluorescence imager: eXplore Optix

Guobin Ma, Pascal Gallant, and Laura McIntosh  »View Author Affiliations


Applied Optics, Vol. 46, Issue 10, pp. 1650-1657 (2007)
http://dx.doi.org/10.1364/AO.46.001650


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Abstract

A key issue in the practical application of fluorescence imaging is the presence of a background signal detected during data acquisition when no target fluorescent material is present. Regardless of the technology employed, background signals cannot be completely eliminated, which limits the detection sensitivity of fluorescence imaging systems, especially for in vivo applications. We present a methodology to characterize the sensitivity of fluorescence imaging devices by taking the background effect into account through the fluorescent signal-to-background ratio (SBR). In an initial application of the methodology, tissuelike liquid phantoms with Cy5.5 fluorescent inclusions were investigated experimentally over a wide range of varying parameters, such as tissue absorption coefficient, scattering coefficient, fluorophore concentration, and inclusion location. By defining detectable and quantifiable SBR thresholds, empirical relations are established, and the sensitivity performance of Advanced Research Technologies's eXplore Optix using Cy5.5 is characterized.

© 2007 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.7050) Medical optics and biotechnology : Turbid media
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Diffuse optical imaging

History
Original Manuscript: July 5, 2006
Revised Manuscript: October 20, 2006
Manuscript Accepted: October 23, 2006
Published: March 13, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Guobin Ma, Pascal Gallant, and Laura McIntosh, "Sensitivity characterization of a time-domain fluorescence imager: eXplore Optix," Appl. Opt. 46, 1650-1657 (2007)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-46-10-1650

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