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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 46, Iss. 10 — Apr. 1, 2007
  • pp: 1735–1741

Polarization effects on scatterer sizing accuracy analyzed with frequency-domain angle-resolved low-coherence interferometry

John W. Pyhtila and Adam Wax  »View Author Affiliations

Applied Optics, Vol. 46, Issue 10, pp. 1735-1741 (2007)

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Angle-resolved low-coherence interferometry (a∕LCI) enables us to make depth-resolved measurements of scattered light that can be used to recover subsurface structural information such as the size of cell nuclei. Endoscopic frequency-domain a∕LCI (fa∕LCI) acquires data by using a novel fiber probe in a fraction of a second, making it a clinically practical system. However, birefringent effects in fiber-based systems can alter the polarization state of the incident light and potentially skew the collected data. We analyze the effect the polarization state of the incident light has on scattering data collected from polystyrene microsphere tissue phantoms and in vitro cell samples and examine the subsequent accuracy of the determined sizes. It is shown that the endoscopic fa∕LCI system accurately determines the size of polystyrene microspheres without the need to control the polarization of the incident beam, but that epithelial cell nuclear sizes are accurately determined only when the polarization state of the incident light is well characterized.

© 2007 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography

ToC Category:
Optical spectroscopic imaging and diagnostics

Original Manuscript: June 22, 2006
Revised Manuscript: September 22, 2006
Manuscript Accepted: September 29, 2006
Published: March 13, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

John W. Pyhtila and Adam Wax, "Polarization effects on scatterer sizing accuracy analyzed with frequency-domain angle-resolved low-coherence interferometry," Appl. Opt. 46, 1735-1741 (2007)

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  1. V. Backman, V. Gopal, M. Kalashnikov, K. Badizadegan, R. Gurjar, A. Wax, I. Georgakoudi, M. Mueller, C. W. Boone, R. R. Dasari, and M. S. Feld, "Measuring cellular structure at submicrometer scale with light scattering spectroscopy," IEEE J. Sel. Top. Quantum Electron. 7, 887-893 (2001). [CrossRef]
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  7. L. B. Lovat, D. Pickard, M. Novelli, P. M. Ripley, H. Francis, I. J. Bigio, and S. G. Bown, "A novel optical biopsy technique using elastic scattering spectroscopy for dysplasia and cancer in Barrett's esophagus," Gastrointest. Endosc. 51, Ab227-Ab227 (2000).
  8. A. Wax, C. H. Yang, M. G. Muller, R. Nines, C. W. Boone, V. E. Steele, G. D. Stoner, R. R. Dasari, and M. S. Feld, "In situ detection of neoplastic transformation and chemopreventive effects in rat esophagus epithelium using angle-resolved low-coherence interferometry," Cancer Res. 63, 3556-3559 (2003). [PubMed]
  9. J. W. Pyhtila, R. N. Graf, and A. Wax, "Determining nuclear morphology using an improved angle-resolved low-coherence interferometry system," Opt. Express 11, 3473-3484 (2003). [CrossRef] [PubMed]
  10. A. Wax, J. W. Pyhtila, R. N. Graf, R. Nines, C. W. Boone, R. R. Dasari, M. S. Feld, V. E. Steele, and G. D. Stoner, "Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometry," J. Biomed. Opt. 10, 051604 (2005). [CrossRef] [PubMed]
  11. J. W. Pyhtila, J. D. Boyer, K. J. Chalut, and A. Wax, "Fourier-domain angle-resolved low-coherence interferometry through an endoscopic fiber bundle for light-scattering spectroscopy," Opt. Lett. 31, 772-774 (2006). [CrossRef] [PubMed]
  12. J. W. Pyhtila and A. Wax, "Rapid, depth-resolved light scattering measurements using Fourier domain, angle-resolved low-coherence interferometry," Opt. Express 12, 6178-6183 (2004). [CrossRef]
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  14. B. E. A. Saleh and M. C. Teich, Fundamentals of Photonics (Wiley, 1991). [CrossRef]
  15. J. W. Pyhtila, H. W. Ma, A. Simnick, A. Chilkoti, and A. Wax, "Analysis of long range correlations due to coherent light scattering from in vitro cell arrays using angle-resolved low coherence interferometry," J. Biomed. Opt. 11, 034022 (2006). [CrossRef] [PubMed]

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