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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 46, Iss. 10 — Apr. 1, 2007
  • pp: 1760–1769

Confocal light absorption and scattering spectroscopic microscopy

Hui Fang, Le Qiu, Edward Vitkin, Munir M. Zaman, Charlotte Andersson, Saira Salahuddin, Lauren M. Kimerer, Patsy B. Cipolloni, Mark D. Modell, Bradley S. Turner, Sarah E. Keates, Irving Bigio, Irving Itzkan, Steven D. Freedman, Rama Bansil, Eugene B. Hanlon, and Lev T. Perelman  »View Author Affiliations


Applied Optics, Vol. 46, Issue 10, pp. 1760-1769 (2007)
http://dx.doi.org/10.1364/AO.46.001760


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Abstract

We have developed a novel optical method for observing submicrometer intracellular structures in living cells, which is called confocal light absorption and scattering spectroscopic (CLASS) microscopy. It combines confocal microscopy, a well-established high-resolution microscopic technique, with light-scattering spectroscopy. CLASS microscopy requires no exogenous labels and is capable of imaging and continuously monitoring individual viable cells, enabling the observation of cell and organelle functioning at scales of the order of 100 nm.

© 2007 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(300.6550) Spectroscopy : Spectroscopy, visible

ToC Category:
Optical spectroscopic imaging and diagnostics

History
Original Manuscript: July 3, 2006
Revised Manuscript: September 13, 2006
Manuscript Accepted: September 15, 2006
Published: March 13, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Hui Fang, Le Qiu, Edward Vitkin, Munir M. Zaman, Charlotte Andersson, Saira Salahuddin, Lauren M. Kimerer, Patsy B. Cipolloni, Mark D. Modell, Bradley S. Turner, Sarah E. Keates, Irving Bigio, Irving Itzkan, Steven D. Freedman, Rama Bansil, Eugene B. Hanlon, and Lev T. Perelman, "Confocal light absorption and scattering spectroscopic microscopy," Appl. Opt. 46, 1760-1769 (2007)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-46-10-1760


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