OSA's Digital Library

Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 46, Iss. 10 — Apr. 1, 2007
  • pp: 1776–1781

Phenotyping transgenic embryonic murine hearts using optical coherence tomography

Michael W. Jenkins, Pankti Patel, Huayun Deng, Monica M. Montano, Michiko Watanabe, and Andrew M. Rollins  »View Author Affiliations

Applied Optics, Vol. 46, Issue 10, pp. 1776-1781 (2007)

View Full Text Article

Enhanced HTML    Acrobat PDF (528 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We used optical coherence tomography (OCT) to characterize the morphological phenotype of embryonic murine hearts discerning hexamethylene-bis-acetamide-inducible protein 1 (HEXIM1) mutants from their wild-type littermates. At E12.5 and E13.5 murine embryos were excised from the mother, the hearts were removed, and 3D OCT data sets were obtained from each heart in the litter. Next, we segmented the morphological borders to obtain cavity volumes and wall thicknesses. The mutant hearts exhibited increased ventricular chamber volume and decreased compact myocardium wall thickness when compared with their wild-type littermates. Also, the E13.5 HEXIM1 −∕− embryo was distinguished by morphological asymmetry (underdeveloped left side).

© 2007 Optical Society of America

OCIS Codes
(100.6890) Image processing : Three-dimensional image processing
(110.4500) Imaging systems : Optical coherence tomography
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography

ToC Category:
Optical Coherence Tomography

Original Manuscript: June 30, 2006
Revised Manuscript: October 26, 2006
Manuscript Accepted: November 17, 2006
Published: March 13, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Michael W. Jenkins, Pankti Patel, Huayun Deng, Monica M. Montano, Michiko Watanabe, and Andrew M. Rollins, "Phenotyping transgenic embryonic murine hearts using optical coherence tomography," Appl. Opt. 46, 1776-1781 (2007)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. W. F. Dietrich, J. Miller, R. Steen, M. A. Merchant, D. Damron-Boles, Z. Husain, R. Dredge, M. J. Daly, K. A. Ingalls, and T. J. O'Connor, "A comprehensive genetic map of the mouse genome," Nature 380, 149-152 (1996). [CrossRef] [PubMed]
  2. B. M. Wittmann, N. Wang, and M. M. Montano, "Identification of a novel inhibitor of breast cell growth that is down-regulated by estrogens and decreased in breast tumors," Cancer Res. 63, 5151-5158 (2003). [PubMed]
  3. B. M. Wittmann, K. Fujinaga, H. Deng, N. Ogba, and M. M. Montano, "The breast cell growth inhibitor, estrogen down regulated gene 1, modulates a novel functional interaction between estrogen receptor alpha and transcriptional elongation factor cyclin T1," Oncogene 24, 5576-5588 (2005). [CrossRef] [PubMed]
  4. H. Deng, Y. Doughman, L. Chaplin, M. Watanabe, and M. M. Montano, "A critical role for HEXIM1 in heart development," FASEB J. 20:A1304-a (2006). [PubMed]
  5. H. Deng, Y. Doughman, L. Chaplin, C. Padro, A. Koch, N. Wang, N. Ward, M. Watanabe, and M. M. Montano are preparing a paper to be called "Disruption of HEXIM1 gene: effects on heart and vascular development."
  6. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991). [CrossRef] [PubMed]
  7. A. F. Fercher, W. Drexler, C. K. Hitzenberger, and T. Lasser, "Optical coherence tomography--principles and applications," Rep. Prog. Phys. 66, 1-239 (2003). [CrossRef]
  8. S. A. Boppart, G. J. Tearney, B. E. Bouma, J. F. Southern, M. E. Brezinski, and J. G. Fujimoto, "Noninvasive assessment of the developing Xenopus cardiovascular system using optical coherence tomograpy," Proc. Natl. Acad. Sci. U.S.A. 94, 4256-4261 (1997). [CrossRef] [PubMed]
  9. T. M. Yelbuz, M. A. Choma, L. Thrane, M. L. Kirby, and J. A. Izatt, "Optical coherence tomography a new high-resolution imaging technology to study cardiac development in chick embryos," Circulation 106, 2771-2774 (2002). [CrossRef] [PubMed]
  10. M. W. Jenkins, F. Rothenberg, D. Roy, Z. Hu, V. P. Nikolski, M. Watanabe, D. L. Wilson, I. R. Efimov, and A. M. Rollins, "4D embryonic cardiography using gated optical coherence tomography," Opt. Express 14, 736-748 (2006). [CrossRef] [PubMed]
  11. W. Luo, D. L. Marks, T. S. Ralston, and S. A. Boppart, "Three-dimensional optical coherence tomography of the embryonic murine cardiovascular system," J. Biomed. Opt. 11, 021014 (2006). [CrossRef] [PubMed]
  12. J. J. Pasquesi, S. C. Schlachter, M. D. Boppart, E. Chaney, S. J. Kaufman, and S. A. Boppart, "In vivo detection of exercise-induced ultrastructural changes in genetically-altered murine skeletal muscle using polarization-sensitive optical coherence tomography," Opt. Express 14, 1547-1556 (2006). [CrossRef] [PubMed]
  13. M. H. Kaufman, The Atlas of Mouse Development (Academic, 1998).
  14. A. M. Rollins, S. Yazdanfar, M. Kulkarni, R. Ung-Arunyawee, and J. Izatt, "In vivo video rate optical coherence tomography," Opt. Express 3, 219-229 (1998). [CrossRef] [PubMed]
  15. Z. Hu and A. M. Rollins, "Quasi-telecentric optical design of a microscope-compatible OCT scanner," Opt. Express 13, 6407-6415 (2005). [CrossRef] [PubMed]
  16. P. Carmeliet, Y. S. Ng, D. Nuyens, G. Theilmeier, K. Brusselmans, I. Cornelissen, E. Ehler, V. V. Kakkar, I. Stalmans, V. Mattot, J. C. Perriard, M. Dewerchin, W. Flameng, A. Nagy, E. Lupu, L. Moons, D. Collen, P. A. D'Amore, and D. T. Shima, "Impaired myocardial angiogenesis and ischemic cardiomyopathy in mice lacking the vascular endothelial growth factor isoforms VEGF164 and VEGF188," Nat. Med. 5, 495-502 (1999). [CrossRef] [PubMed]
  17. D. Abraham, R. Hofbauer, R. Schafer, R. Blumer, P. Paulus, A. Miksovsky, H. Traxler, A. Kocher, and S. Aharinejad, "Selective downregulation of VEGF-A(165), VEGF-R(1), and decreased capillary density in patients with dilative but not ischemic cardiomyopathy," Circ. Res. 87, 644-647 (2000). [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3
Fig. 4

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited