Phenotyping transgenic embryonic murine hearts using optical coherence tomography
Applied Optics, Vol. 46, Issue 10, pp. 1776-1781 (2007)
http://dx.doi.org/10.1364/AO.46.001776
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Abstract
We used optical coherence tomography (OCT) to characterize the morphological phenotype of embryonic murine hearts discerning hexamethylene-bis-acetamide-inducible protein 1 (HEXIM1) mutants from their wild-type littermates. At E12.5 and E13.5 murine embryos were excised from the mother, the hearts were removed, and 3D OCT data sets were obtained from each heart in the litter. Next, we segmented the morphological borders to obtain cavity volumes and wall thicknesses. The mutant hearts exhibited increased ventricular chamber volume and decreased compact myocardium wall thickness when compared with their wild-type littermates. Also, the E13.5 HEXIM1 −∕− embryo was distinguished by morphological asymmetry (underdeveloped left side).
© 2007 Optical Society of America
OCIS Codes
(100.6890) Image processing : Three-dimensional image processing
(110.4500) Imaging systems : Optical coherence tomography
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
ToC Category:
Optical Coherence Tomography
History
Original Manuscript: June 30, 2006
Revised Manuscript: October 26, 2006
Manuscript Accepted: November 17, 2006
Published: March 13, 2007
Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics
Citation
Michael W. Jenkins, Pankti Patel, Huayun Deng, Monica M. Montano, Michiko Watanabe, and Andrew M. Rollins, "Phenotyping transgenic embryonic murine hearts using optical coherence tomography," Appl. Opt. 46, 1776-1781 (2007)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-46-10-1776
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