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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 46, Iss. 10 — Apr. 1, 2007
  • pp: 1843–1851

Confocal theta line-scanning microscope for imaging human tissues

Peter J. Dwyer, Charles A. DiMarzio, and Milind Rajadhyaksha  »View Author Affiliations


Applied Optics, Vol. 46, Issue 10, pp. 1843-1851 (2007)
http://dx.doi.org/10.1364/AO.46.001843


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Abstract

A confocal reflectance theta line-scanning microscope demonstrates imaging of nuclear and cellular morphology in human skin and oral mucosa in vivo. The illumination and detection are through a divided objective lens pupil, resulting in a theta-microscope configuration. A line is directly scanned in the pupil and descanned onto a linear detector array such that the theta line scanner consists of only seven main optical components. The experimentally measured lateral resolution is 1.0   μm and optical section thickness is 1.7   μm under nominal conditions at 830   nm wavelength. Through full-thickness human epidermis (i.e., in the dermis) the measured lateral resolution is 1.7   μm and the optical section thickness is 9.2   μm . The lateral resolution, sectioning, and image quality in epidermal (epithelial) tissue is comparable to that of point scanning confocal microscopy.

© 2007 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Optical microscopy techniques and technology

History
Original Manuscript: June 29, 2006
Revised Manuscript: November 27, 2006
Manuscript Accepted: December 8, 2006
Published: March 13, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Peter J. Dwyer, Charles A. DiMarzio, and Milind Rajadhyaksha, "Confocal theta line-scanning microscope for imaging human tissues," Appl. Opt. 46, 1843-1851 (2007)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-46-10-1843


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