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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 46, Iss. 10 — Apr. 1, 2007
  • pp: 1860–1865

Random access three-dimensional two-photon microscopy

Balázs Rózsa, Gergely Katona, E. Sylvester Vizi, Zoltán Várallyay, Attila Sághy, Lásló Valenta, Pál Maák, Júlia Fekete, Ákos Bányász, and Róbert Szipőcs  »View Author Affiliations


Applied Optics, Vol. 46, Issue 10, pp. 1860-1865 (2007)
http://dx.doi.org/10.1364/AO.46.001860


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Abstract

We propose a two-photon microscope scheme capable of real-time, three-dimensional investigation of the electric activity pattern of neural networks or signal summation rules of individual neurons in a 0.6 m m × 0.6 m m × 0.2 m m volume of the sample. The points of measurement are chosen according to a conventional scanning two-photon image, and they are addressed by separately adjustable optical fibers. This allows scanning at kilohertz repetition rates of as many as 100 data points. Submicrometer spatial resolution is maintained during the measurement similarly to conventional two-photon microscopy.

© 2007 Optical Society of America

OCIS Codes
(110.2350) Imaging systems : Fiber optics imaging
(110.6880) Imaging systems : Three-dimensional image acquisition
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology

ToC Category:
Optical microscopy techniques and technology

History
Original Manuscript: July 5, 2006
Revised Manuscript: December 21, 2006
Manuscript Accepted: December 21, 2006
Published: March 13, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Balázs Rózsa, Gergely Katona, E. Sylvester Vizi, Zoltán Várallyay, Attila Sághy, Lásló Valenta, Pál Maák, Júlia Fekete, Ákos Bányász, and Róbert Szipőcs, "Random access three-dimensional two-photon microscopy," Appl. Opt. 46, 1860-1865 (2007)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-46-10-1860

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