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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 46, Iss. 10 — Apr. 1, 2007
  • pp: 1890–1895

Oblique-incidence reflectivity difference microscope for label-free high-throughput detection of biochemical reactions in a microarray format

Xiangdong Zhu, James P. Landry, Yung-Shin Sun, Jeff P. Gregg, Kit S. Lam, and Xiaowen Guo  »View Author Affiliations


Applied Optics, Vol. 46, Issue 10, pp. 1890-1895 (2007)
http://dx.doi.org/10.1364/AO.46.001890


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Abstract

We describe a recently developed oblique-incidence reflectivity difference (OI-RD) microscope, a form of polarization-modulated imaging ellipsometer, for label-free–high-throughput detection of biomolecular reactions on DNA and protein microarrays. We present examples of application of this technique to end-point and real-time investigations of DNA–DNA hybridization, antibody–antigen capture, and protein–small-molecule binding reactions. Compared to a conventional imaging ellipsometer based on the polarizer–compensator–sample–analyzer scheme and under the off-null condition, a polarization-modulated OI-RD microscope is inherently more sensitive by at least 1 order of magnitude to thickness changes on a solid surface. Compared with imaging surface plasmon resonance microscopes based on reflectance change on falling or rising slopes of the surface plasmon resonance, the OI-RD microscope (1) has a comparable sensitivity, (2) is applicable to conventional microscope glass slides, and (3) easily covers a field of view as large as the entire surface of a 1   in . × 3   in . ( 2.54 c m × 7.62 c m ) microscope slide.

© 2007 Optical Society of America

OCIS Codes
(240.0240) Optics at surfaces : Optics at surfaces
(310.0310) Thin films : Thin films

ToC Category:
Optics in molecular imaging and drug discovery

History
Original Manuscript: June 29, 2006
Revised Manuscript: September 1, 2006
Manuscript Accepted: September 15, 2006
Published: March 13, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Xiangdong Zhu, James P. Landry, Yung-Shin Sun, Jeff P. Gregg, Kit S. Lam, and Xiaowen Guo, "Oblique-incidence reflectivity difference microscope for label-free high-throughput detection of biochemical reactions in a microarray format," Appl. Opt. 46, 1890-1895 (2007)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-46-10-1890


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References

  1. M. Schena, Microarray Analysis (Wiley, 2003).
  2. H. Zhu, M. Bilgin, R. Bangham, D. Hall, A. Casamayor, P. Bertone, Ning Lan, R. Jansen, S. Bidlingmaier, T. Moufek, T. Mitchell, P. Miller, R. A. Dean, M. Gerstein, and M. Scheider, "Global analysis of protein activities using proteome chips," Science 293, 2101-2105 (2001). [CrossRef] [PubMed]
  3. G. MacBeath, "Protein microarrays and proteomics," Nat. Genet. 32, 526-532 (2002). [CrossRef] [PubMed]
  4. T. Lindahl and R. D. Wood, "Quality control by DNA repair," Science 286, 1897-1905 (1999). [CrossRef] [PubMed]
  5. B. P. Nelson, A. G. Frutos, J. M. Brockman, and R. M. Corn, "Near-infrared surface plasmon resonance measurements of ultrathin films. 1. Angle shift and SPR imaging experiments," Anal. Chem. 71, 3928-2934 (1999). [CrossRef]
  6. J. S. Shumaker-Parry and C. T. Campbell, "Quantitative methods for spatially resolved adsorption/desorption measurements in real time by surface plasmon resonance microscopy," Anal. Chem. 76, 907-917 (2004). [CrossRef] [PubMed]
  7. L. S. Jung, C. T. Campbell, T. M. Chinowsky, M. N. Mar, and S. S. Yee, "Quantitative interpretation of the response of surface plasmon resonance sensors to adsorbed films," Langmuir 14, 5636-5648 (1998). [CrossRef]
  8. R. M. A. Azzam and N. M. Bashara, Ellipsometry and Polarized Light (Elsevier Science, 1987).
  9. G. Jin, R. Jansson, and H. Arwin, "Imaging ellipsometry revisited: development for visualization of thin transparent layers on silicon substrates," Rev. Sci. Instrum. 67, 2930-2936 (1996). [CrossRef]
  10. Z. H. Wang and G. Jin, "A label-free multi-sensing immuno-sensor based on imaging ellipsometry," Anal. Chem. 75, 6119-6123 (2003). [CrossRef] [PubMed]
  11. J. P. Landry, J. P. Gregg, and X. D. Zhu, "Label-free detection of microarrays of biomolecules by oblique-incidence reflectivity difference microscopy," Opt. Lett. 29, 581-583 (2004). [CrossRef] [PubMed]
  12. J. Piehler, A. Brecht, and G. Gauglitz, "Affinity detection of low molecular weight analytes," Anal. Chem. 68, 139-143 (1996). [CrossRef] [PubMed]
  13. P. Thomas, E. Nabighian, M. C. Bartelt, C. Y. Fong, and X. D. Zhu, "An oblique-incidence optical reflectivity difference and LEED study of rare-gas growth on a lattice-mismatched metal substrate," Appl. Phys. A 79, 131-137 (2004). [CrossRef]
  14. Y. Y. Fei, X. D. Zhu, L. F. Liu, H. B. Liu, Z. H. Chen, and G. Z. Yang, "Oscillations in oblique-incidence optical reflection from a growth surface during layer-by-layer epitaxy," Phys. Rev. B 69, 233405 (2004). [CrossRef]
  15. W. Schwarzacher, J. Gray, and X. D. Zhu, "Oblique incidence reflectivity difference as an in situ probe of electrodeposition: Co on Au," Electrochem. Solid-State Lett. 6, C73-C76 (2003). [CrossRef]
  16. X. D. Zhu, "Oblique-incidence optical reflectivity difference from a rough film of crystalline material," Phys. Rev. B 69, 115407 (2004). [CrossRef]
  17. J. P. Landry, J. Gray, M. K. O'Toole, and X. D. Zhu, "Incidence-angle dependence of optical reflectivity difference from an ultrathin film on solid surface," Opt. Lett. 31, 531-533 (2006). [CrossRef] [PubMed]
  18. B. Liedberg, C. Nylander, and I. Lundstrom, "Surface plasmon resonance for gas detection and biosensing," Sens. Actuators 4, 299-304 (1983). [CrossRef]
  19. X. D. Zhu, "Comparison of two optical techniques for label-free detection of biomolecular microarrays on solids," Opt. Commun. 259, 751-753 (2006). [CrossRef]
  20. W. A. Hendrickson, A. Paehler, J. L. Smith, Y. Satow, E. A. Merritt, and R. P. Phizackerley, "Crystal structure of core streptavidin determined from multiwavelength anomalous diffraction of synchrotron radiation," Proc. Natl. Acad. Sci. USA 86, 2190-2194 (1989). [CrossRef] [PubMed]
  21. P. Weber, D. Ohlendorf, J. Wendoloski, and F. Salemme, "Structural origins of high-affinity biotin binding to streptavidin," Science 243, 85-88 (1989). [CrossRef] [PubMed]

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