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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 46, Iss. 10 — Apr. 1, 2007
  • pp: 1918–1923

Strategy for photostable proximity bioassays using lanthanides

Jeanne P. Haushalter and Gregory W. Faris  »View Author Affiliations

Applied Optics, Vol. 46, Issue 10, pp. 1918-1923 (2007)

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We report initial findings for research aimed at creating photostable lanthanide chelate reporters for proximity assays. These reporters take advantage of the nanometer-scale distance dependence of fluorescence enhancement for molecules in the vicinity of noble metal nanoparticles and also capitalize on some unique properties of lanthanide chelates. This approach promises to lead to proximity assays that do not suffer from photobleaching and offer very high on∕off enhancement ratios. Results for lanthanide chelates on silver island films and in colloidal suspensions are reported. Enhancement factors range from 1 to 2 orders of magnitude, with larger enhancements for strongly quenched lanthanides.

© 2007 Optical Society of America

OCIS Codes
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(240.6680) Optics at surfaces : Surface plasmons

ToC Category:
Optical nanotechnology

Original Manuscript: June 30, 2006
Revised Manuscript: October 12, 2006
Manuscript Accepted: October 23, 2006
Published: March 13, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Jeanne P. Haushalter and Gregory W. Faris, "Strategy for photostable proximity bioassays using lanthanides," Appl. Opt. 46, 1918-1923 (2007)

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