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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 46, Iss. 11 — Apr. 10, 2007
  • pp: 2131–2137

Analytical method for localizing a fluorescent inclusion in a turbid medium

A. Laidevant, A. Da Silva, M. Berger, J. Boutet, J.-M. Dinten, and A. C. Boccara  »View Author Affiliations

Applied Optics, Vol. 46, Issue 11, pp. 2131-2137 (2007)

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We describe a novel method for localizing a fluorescent inclusion in a homogeneous turbid medium through the use of time-resolved techniques. Based on the calculation of the mean time of the fluorescence curves, the method does not require a priori knowledge of either the fluorescence lifetime or the mean time of the instrument response function since it adopts a differential processing approach. Theoretical expressions were validated and experiments for assessing the accuracy of localization were carried out on liquid optical phantoms with a small fluorescent inclusion. The illumination and detection optical fibers were immersed in the medium to achieve infinite medium geometry as required by the model used. The experimental setup consisted of a time-correlated single-photon counting system. Submillimeter accuracy was achieved for the localization of the inclusion.

© 2007 Optical Society of America

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5280) Medical optics and biotechnology : Photon migration
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(170.7050) Medical optics and biotechnology : Turbid media
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: August 21, 2006
Revised Manuscript: October 27, 2006
Manuscript Accepted: October 28, 2006
Published: March 20, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

A. Laidevant, A. Da Silva, M. Berger, J. Boutet, J.-M. Dinten, and A. C. Boccara, "Analytical method for localizing a fluorescent inclusion in a turbid medium," Appl. Opt. 46, 2131-2137 (2007)

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