We demonstrate the effectiveness of laser-induced fluorescence (LIF) for monitoring the development and stress detection of in vitro tissue cultures in a nondestructive and noninvasive way. The changes in LIF spectra caused by the induction of organogenesis, the increase of the F690∕F740 ratio as a result of the stress originated in the organogenic explants due to shoot emergence, and the relationship between fluorescence spectra and shoot development were detected by LIF through closed containers of Saintpaulia ionantha.
© 2007 Optical Society of America
Original Manuscript: January 27, 2006
Revised Manuscript: September 12, 2006
Manuscript Accepted: November 6, 2006
Published: March 20, 2007
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics
Ana Celia Muñoz-Muñoz, Humberto Gutiérrez-Pulido, José Manuel Rodríguez-Domínguez, Antonia Gutiérrez-Mora, Benjamín Rodríguez-Garay, and Jesús Cervantes-Martínez, "Analysis of laser-induced fluorescence spectra of in vitro plant tissue cultures," Appl. Opt. 46, 2138-2142 (2007)