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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 46, Iss. 14 — May. 10, 2007
  • pp: 2769–2777

Experimental prediction of the wavelength-dependent path-length factor for optical intrinsic signal analysis

Koichiro Sakaguchi, Tomoya Tachibana, Shunsuke Furukawa, Takushige Katsura, Kyoko Yamazaki, Hideo Kawaguchi, Atsushi Maki, and Eiji Okada  »View Author Affiliations

Applied Optics, Vol. 46, Issue 14, pp. 2769-2777 (2007)

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Analysis of the optical intrinsic signal of an exposed cortex has been applied to measurement of functional brain activation. It is important for accurate measurement of concentration changes in oxygenated hemoglobin and deoxygenated hemoglobin to consider the wavelength dependence of the mean optical path lengths for the reflectance of cortical tissue. A method is proposed to experimentally estimate the wavelength dependence of the mean optical path length in cortical tissue from the multispectral reflectance of the exposed cortex without any additional instruments. The trend in the wavelength dependence of the mean optical path length estimated by the proposed method agrees with that estimated by the model-based prediction, whereas the magnitude of the wavelength dependence predicted by the proposed method is greater than that of the model-based prediction. The experimentally predicted mean optical path length minimizes the difference in the measured changes in the concentrations of the oxygenated hemoglobin and deoxygenated hemoglobin calculated from different wavelength pairs.

© 2007 Optical Society of America

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5280) Medical optics and biotechnology : Photon migration
(300.6550) Spectroscopy : Spectroscopy, visible

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 3, 2006
Revised Manuscript: November 24, 2006
Manuscript Accepted: November 25, 2006
Published: April 23, 2007

Virtual Issues
Vol. 2, Iss. 6 Virtual Journal for Biomedical Optics

Koichiro Sakaguchi, Tomoya Tachibana, Shunsuke Furukawa, Takushige Katsura, Kyoko Yamazaki, Hideo Kawaguchi, Atsushi Maki, and Eiji Okada, "Experimental prediction of the wavelength-dependent path-length factor for optical intrinsic signal analysis," Appl. Opt. 46, 2769-2777 (2007)

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