OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 46, Iss. 17 — Jun. 10, 2007
  • pp: 3617–3627

Noncontact optical imaging in mice with full angular coverage and automatic surface extraction

Heiko Meyer, Anikitos Garofalakis, Giannis Zacharakis, Stylianos Psycharakis, Clio Mamalaki, Dimitris Kioussis, Eleftherios N. Economou, Vasilis Ntziachristos, and Jorge Ripoll  »View Author Affiliations


Applied Optics, Vol. 46, Issue 17, pp. 3617-3627 (2007)
http://dx.doi.org/10.1364/AO.46.003617


View Full Text Article

Enhanced HTML    Acrobat PDF (1336 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

During the past decade, optical imaging combined with tomographic approaches has proved its potential in offering quantitative three-dimensional spatial maps of chromophore or fluorophore concentration in vivo. Due to its direct application in biology and biomedicine, diffuse optical tomography (DOT) and its fluorescence counterpart, fluorescence molecular tomography (FMT), have benefited from an increase in devoted research and new experimental and theoretical developments, giving rise to a new imaging modality. The most recent advances in FMT and DOT are based on the capability of collecting large data sets by using CCDs as detectors, and on the ability to include multiple projections through recently developed noncontact approaches. For these to be implemented, we have developed an imaging setup that enables three-dimensional imaging of arbitrary shapes in fluorescence or absorption mode that is appropriate for small animal imaging. This is achieved by implementing a noncontact approach both for sources and detectors and coregistering surface geometry measurements using the same CCD camera. A thresholded shadowgrammetry approach is applied to the geometry measurements to retrieve the surface mesh. We present the evaluation of the system and method in recovering three-dimensional surfaces from phantom data and live mice. The approach is used to map the measured in vivo fluorescence data onto the tissue surface by making use of the free-space propagation equations, as well as to reconstruct fluorescence concentrations inside highly scattering tissuelike phantom samples. Finally, the potential use of this setup for in vivo small animal imaging and its impact on biomedical research is discussed.

© 2007 Optical Society of America

OCIS Codes
(040.1520) Detectors : CCD, charge-coupled device
(100.3010) Image processing : Image reconstruction techniques
(110.0110) Imaging systems : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Imaging Systems

History
Original Manuscript: June 16, 2006
Revised Manuscript: January 31, 2007
Manuscript Accepted: February 9, 2007
Published: May 18, 2007

Virtual Issues
Vol. 2, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Heiko Meyer, Anikitos Garofalakis, Giannis Zacharakis, Stylianos Psycharakis, Clio Mamalaki, Dimitris Kioussis, Eleftherios N. Economou, Vasilis Ntziachristos, and Jorge Ripoll, "Noncontact optical imaging in mice with full angular coverage and automatic surface extraction," Appl. Opt. 46, 3617-3627 (2007)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-46-17-3617


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. N. C. Shaner, R. E. Campbell, P. A. Steinbach, B. N. G. Giepmans, A. E. Palmer, and R. Y. Tsien, "Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent proteins," Nat. Biotechnol. 22, 1567-1572 (2004). [CrossRef] [PubMed]
  2. R. Y. Tsien, "The green fluorescent protein," Annu. Rev. Biochem. 67, 509-544 (1998). [CrossRef] [PubMed]
  3. R. Weissleder and V. Ntziachristos, "Shedding light onto live molecular targets," Nat. Med. 9, 123-128 (2003). [CrossRef] [PubMed]
  4. V. Ntziachristos, C. Bremer, E. E. Graves, J. Ripoll, and R. Weissleder, "In vivo tomographic imaging of near-infrared fluorescent probes," Molecular Imaging 1, 82-88 (2002). [CrossRef] [PubMed]
  5. V. Ntziachristos, J. Ripoll, L. V. Wang, and R. Weissleder, "Looking and listening to light: the evolution of whole-body photonic imaging," Nat. Biotechnol. 23, 313-320 (2005). [CrossRef] [PubMed]
  6. V. Ntziachristos, E. A. Schellenberger, J. Ripoll, D. Yessayan, E. Graves, A. Bogdanov, Jr., L. Josephson, and R. Weissleder, "Visualization of antitumor treatment by means of fluorescence molecular tomography with an annexin V-Cy5.5 conjugate," Proc. Natl. Acad. Sci. U.S.A. 101, 12294-12299 (2004). [CrossRef] [PubMed]
  7. V. Ntziachristos, C. Tung, C. Bremer, and R. Weissleder, "Fluorescence molecular tomography resolves protease activity in vivo," Nat. Med. 8, 757-760 (2002). [CrossRef] [PubMed]
  8. R. M. Hoffman, "Green fluorescent protein imaging of tumour growth, metastasis, and angiogenesis in mouse models," Lancet Oncology 3, 546-556 (2002). [CrossRef] [PubMed]
  9. E. M. Hillman, J. C. Hebden, M. Schweiger, H. Dehghani, F. E. Schmidt, D. T. Delpy, and S. R. Arridge, "Time resolved optical tomography of the human forearm," Phys. Med. Biol. 46, 1117-1130 (2001). [CrossRef] [PubMed]
  10. J. C. Hebden, A. Gibson, T. Austin, R. M. Yusof, N. Everdell, D. T. Delpy, S. R. Arridge, J. H. Meek, and J. S. Wyatt, "Imaging changes in blood volume and oxygenation in the newborn infant brain using three-dimensional optical tomography," Phys. Med. Biol. 49, 1117-1130 (2004). [CrossRef] [PubMed]
  11. V. Ntziachristos and R. Weissleder, "Charge-coupled-based scanner for tomography of fluorescent near-infrared probes in turbid media," Med. Phys. 29, 803-809 (2002). [CrossRef] [PubMed]
  12. G. Zacharakis, J. Ripoll, R. Weissleder, and V. Ntziachristos, "Fluorescent protein tomography scanner for small animal imaging," IEEE Trans. Med. Imaging 24, 878-885 (2005). [CrossRef] [PubMed]
  13. J. Culver, V. Ntziachristos, M. Holboke, and A. Yodh, "Optimization of optode arrangements for diffuse optical tomography: A singular value analysis," Opt. Lett. 26, 701-703 (2001). [CrossRef] [PubMed]
  14. E. E. Graves, J. P. Culver, J. Ripoll, R. Weissleder, and V. Ntziachristos, "Singular-value analysis and optimization of experimental parameters in fluorescence molecular tomography," J. Opt. Soc. Am. A 21, 231-241 (2004). [CrossRef] [PubMed]
  15. V. A. Markel and J. C. Schotland, "Symmetries, inversion formulas, and image reconstruction for optical tomography," Phys. Rev. E 70, 056616 (2004). [CrossRef] [PubMed]
  16. G. M. Turner, G. Zacharakis, A. Soubret, J. Ripoll, and V. Ntziachristos, "Complete-angle projection diffuse optical tomography by use of early photons," Opt. Lett. 30, 409-411 (2005). [CrossRef] [PubMed]
  17. J. Ripoll, R. B. Schulz, and V. Ntziachristos, "Free-space propagation of diffuse light: theory and experiments," Phys. Rev. Lett. 91, 103901 (2003). [CrossRef] [PubMed]
  18. R. B. Schulz, J. Ripoll, and V. Ntziachristos, "Noncontact optical tomography of turbid media," Opt. Lett. 28, 1701-1703 (2003). [CrossRef] [PubMed]
  19. R. B. Schulz, J. Ripoll, and V. Ntziachristos, "Experimental fluorescence tomography of tissues with noncontact measurements," IEEE Trans. Med. Imaging 23, 492-500 (2004). [CrossRef] [PubMed]
  20. J. Sharpe, "Optical projection tomography," Annu. Rev. Biomed. Eng. 6, 209-228 (2004). [CrossRef] [PubMed]
  21. J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, "Optical projection tomography as a tool for 3D microscopy and gene expression studies," Science 296, 541-545 (2002). [CrossRef] [PubMed]
  22. XENOGEN, U.S. patent application 20060268153, (6 November 2006).
  23. D. J. Lee, X. Q. Xu, J. Eifert, and P. C. Zhan, "Area and volume measurements of objects with irregluar shapes using multiple silhouettes," Opt. Eng. 45, 027202 (2006). [CrossRef] [PubMed]
  24. R. T. Whitaker and V. Elangovan, "A direct approach to estimating surfaces in tomographic data," Med. Image Anal. 6, 235-249 (2002). [CrossRef] [PubMed]
  25. G. Tognola, M. Parazzini, C. Svelto, P. Ravazzani, and F. Grandori, "A fast and reliable system for 3D surface acquisition and reconstruction," Image Vis. Comput. 21, 295-305 (2003). [CrossRef] [PubMed]
  26. D. Rypl and P. Krysl, "Triangulation of 3D surfaces," Eng. Comput. 13, 87-98 (1997). [CrossRef]
  27. J. Ripoll, M. Nieto-Vesperinas, R. Weissleder, and V. Ntziachristos, "Fast analytical approximation for arbitrary geometries in diffuse optical tomography," Opt. Lett. 27, 527-529 (2002). [CrossRef]
  28. J. Ripoll and V. Ntziachristos, "Iterative boundary method for diffuse optical tomography," J. Opt. Soc. Am. A 20, 1103-1110 (2003). [CrossRef] [PubMed]
  29. A. Kak and M. Slaney, Principles of Computerized Tomographic Imaging (IEEE, 1988). [PubMed]
  30. J. R. Walls, J. G. Sled, J. Sharpe, and R. M. Henkelman, "Correction of artefacts in optical projection tomography," Phys. Med. Biol. 50, 4645-4665 (2005). [CrossRef] [PubMed]
  31. B. W. Rice, H. Xu, and C. Kuo, "Surface construction using combined photographic and structured light information," U.S. patent application 20060268153 (4 May 2006).
  32. J. de Boer, A. Williams, G. Skavdis, N. Harker, M. Coles, M. Tolaini, T. Norton, K. Williams, K. Roderick, A. Potocnik, and D. Kioussis, "Transgenic mice with hematopoeitic and lymphoid specific expression of Cre," Eur. J. Immunol. 33, 314-325 (2003). [CrossRef] [PubMed]
  33. V. Ntziachristos and R. Weissleder, "Experimental three-dimensional fluorescence reconstruction of diffuse media using a normalized Born approximation," Opt. Lett. 26, 893-895 (2001). [CrossRef] [PubMed]
  34. J. Ripoll, V. Ntziachristos, R. Carminati, and M. Nieto-Vesperinas, "The Kirchhoff approximation for diffusive waves," Phys. Rev. E 64, 051917 (2001). [CrossRef] [PubMed]
  35. J. Ripoll and V. Ntziachristos, "Imaging scattering media from a distance: theory and applications of non-contact optical tomography," Mod. Phys. Lett. B 18, 1403-1431 (2004). [CrossRef]
  36. A. Ishimaru, Wave Propagation and Scattering in Random Media (Academic, 1978), Vol. 1.
  37. M. Born and E. Wolf, Principles of Optics, 7th ed. (Cambridge U. Press, 1999). [PubMed]
  38. A. Soubret, J. Ripoll, and V. Ntziachristos, "Accuracy of fluorescent tomography in the presence of heterogeneities: study of the normalized Born ratio," IEEE Trans. Med. Imaging 24, 1377-1386 (2005). [CrossRef] [PubMed]
  39. A. Soubret and V. Ntziachristos, "Fluorescence molecular tomography in the presence of background fluorescence," Phys. Med. Biol. 51, 3983-4001 (2006). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited