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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 46, Iss. 17 — Jun. 10, 2007
  • pp: 3617–3627

Noncontact optical imaging in mice with full angular coverage and automatic surface extraction

Heiko Meyer, Anikitos Garofalakis, Giannis Zacharakis, Stylianos Psycharakis, Clio Mamalaki, Dimitris Kioussis, Eleftherios N. Economou, Vasilis Ntziachristos, and Jorge Ripoll  »View Author Affiliations

Applied Optics, Vol. 46, Issue 17, pp. 3617-3627 (2007)

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During the past decade, optical imaging combined with tomographic approaches has proved its potential in offering quantitative three-dimensional spatial maps of chromophore or fluorophore concentration in vivo. Due to its direct application in biology and biomedicine, diffuse optical tomography (DOT) and its fluorescence counterpart, fluorescence molecular tomography (FMT), have benefited from an increase in devoted research and new experimental and theoretical developments, giving rise to a new imaging modality. The most recent advances in FMT and DOT are based on the capability of collecting large data sets by using CCDs as detectors, and on the ability to include multiple projections through recently developed noncontact approaches. For these to be implemented, we have developed an imaging setup that enables three-dimensional imaging of arbitrary shapes in fluorescence or absorption mode that is appropriate for small animal imaging. This is achieved by implementing a noncontact approach both for sources and detectors and coregistering surface geometry measurements using the same CCD camera. A thresholded shadowgrammetry approach is applied to the geometry measurements to retrieve the surface mesh. We present the evaluation of the system and method in recovering three-dimensional surfaces from phantom data and live mice. The approach is used to map the measured in vivo fluorescence data onto the tissue surface by making use of the free-space propagation equations, as well as to reconstruct fluorescence concentrations inside highly scattering tissuelike phantom samples. Finally, the potential use of this setup for in vivo small animal imaging and its impact on biomedical research is discussed.

© 2007 Optical Society of America

OCIS Codes
(040.1520) Detectors : CCD, charge-coupled device
(100.3010) Image processing : Image reconstruction techniques
(110.0110) Imaging systems : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Imaging Systems

Original Manuscript: June 16, 2006
Revised Manuscript: January 31, 2007
Manuscript Accepted: February 9, 2007
Published: May 18, 2007

Virtual Issues
Vol. 2, Iss. 7 Virtual Journal for Biomedical Optics

Heiko Meyer, Anikitos Garofalakis, Giannis Zacharakis, Stylianos Psycharakis, Clio Mamalaki, Dimitris Kioussis, Eleftherios N. Economou, Vasilis Ntziachristos, and Jorge Ripoll, "Noncontact optical imaging in mice with full angular coverage and automatic surface extraction," Appl. Opt. 46, 3617-3627 (2007)

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