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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 46, Iss. 17 — Jun. 10, 2007
  • pp: 3639–3648

Biophysical modeling of forward scattering from bacterial colonies using scalar diffraction theory

Euiwon Bae, Padmapriya P. Banada, Karleigh Huff, Arun K. Bhunia, J. Paul Robinson, and E. Daniel Hirleman  »View Author Affiliations

Applied Optics, Vol. 46, Issue 17, pp. 3639-3648 (2007)

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A model for forward scattering from bacterial colonies is presented. The colonies of interest consist of approximately 10 12 10 13 individual bacteria densely packed in a configuration several millimeters in diameter and approximately 0.1 0.2 mm in thickness. The model is based on scalar diffraction theory and accounts for amplitude and phase modulation created by three macroscopic properties of the colonies: phase modulation due to the surface topography, phase modulation due to the radial structure observed from some strains and species, and diffraction from the outline of the colony. Phase contrast and confocal microscopy were performed to provide quantitative information on the shape and internal structure of the colonies. The computed results showed excellent agreement with the experimental scattering data for three different Listeria species: Listeria innocua, Listeria ivanovii, and Listeria monocytogenes. The results provide a physical explanation for the unique and distinctive scattering signatures produced by colonies of closely related Listeria species and support the efficacy of forward scattering for rapid detection and classification of pathogens without tagging.

© 2007 Optical Society of America

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(290.0290) Scattering : Scattering

ToC Category:
Instrumentation, Measurement, and Metrology

Original Manuscript: November 22, 2006
Revised Manuscript: February 7, 2007
Manuscript Accepted: February 8, 2007
Published: May 18, 2007

Virtual Issues
Vol. 2, Iss. 7 Virtual Journal for Biomedical Optics

Euiwon Bae, Padmapriya P. Banada, Karleigh Huff, Arun K. Bhunia, J. Paul Robinson, and E. Daniel Hirleman, "Biophysical modeling of forward scattering from bacterial colonies using scalar diffraction theory," Appl. Opt. 46, 3639-3648 (2007)

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