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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 46, Iss. 19 — Jul. 1, 2007
  • pp: 3969–3975

Measurement of acetaldehyde in exhaled breath using a laser absorption spectrometer

Pratyuma C. Kamat, Chad B. Roller, Khosrow Namjou, James D. Jeffers, Ali Faramarzalian, Rodolfo Salas, and Patrick J. McCann  »View Author Affiliations

Applied Optics, Vol. 46, Issue 19, pp. 3969-3975 (2007)

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A high-resolution liquid-nitrogen-free mid-infrared tunable diode laser absorption spectroscopy (TDLAS) system was used to perform real-time measurement of acetaldehyde concentrations in human exhaled breath following ingestion of an alcoholic beverage. Acetaldehyde absorption features were measured near 5.79 μm ( 1727 cm 1 ) using a IV–VI semiconductor laser, a 100   m long path optical gas cell, and second- harmonic detection coupled with wavelength modulation. Acetaldehyde levels were measured with a minimum detection limit of 80 ppb for 5 s integration time. The variations in exhaled acetaldehyde levels over time were analyzed prior to and following ingestion of two different amounts of white wine. A method to calibrate acetaldehyde measurements internally using water vapor absorption lines was investigated to eliminate the need for system calibration with gas standards. The potential of a TDLAS system to be used as a noninvasive clinical tool for measurements of large volatile compounds with possible applications in cancer detection is demonstrated.

© 2007 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(140.3070) Lasers and laser optics : Infrared and far-infrared lasers
(300.1030) Spectroscopy : Absorption
(300.6340) Spectroscopy : Spectroscopy, infrared

ToC Category:
Absorption and Cavity-Based Techniques

Original Manuscript: October 16, 2006
Manuscript Accepted: December 13, 2006
Published: June 12, 2007

Virtual Issues
Vol. 2, Iss. 8 Virtual Journal for Biomedical Optics

Pratyuma C. Kamat, Chad B. Roller, Khosrow Namjou, James D. Jeffers, Ali Faramarzalian, Rodolfo Salas, and Patrick J. McCann, "Measurement of acetaldehyde in exhaled breath using a laser absorption spectrometer," Appl. Opt. 46, 3969-3975 (2007)

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