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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 46, Iss. 22 — Aug. 1, 2007
  • pp: 4999–5008

Fourier-domain digital holographic optical coherence imaging of living tissue

Kwan Jeong, John J. Turek, and David D. Nolte  »View Author Affiliations

Applied Optics, Vol. 46, Issue 22, pp. 4999-5008 (2007)

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Digital holographic optical coherence imaging is a full-frame coherence-gated imaging approach that uses a CCD camera to record and reconstruct digital holograms from living tissue. Recording digital holograms at the optical Fourier plane has advantages for diffuse targets compared with Fresnel off-axis digital holography. A digital hologram captured at the Fourier plane requires only a 2D fast Fourier transform for numerical reconstruction. We have applied this technique for the depth-resolved imaging of rat osteogenic tumor multicellular spheroids and acquired cross-section images of the anterior segment and the retinal region of a mouse eye. A penetration depth of 1.4   mm for the tumor spheroids was achieved.

© 2007 Optical Society of America

OCIS Codes
(070.2590) Fourier optics and signal processing : ABCD transforms
(090.1760) Holography : Computer holography
(110.6150) Imaging systems : Speckle imaging
(170.1650) Medical optics and biotechnology : Coherence imaging
(170.7050) Medical optics and biotechnology : Turbid media

ToC Category:
Imaging Systems

Original Manuscript: December 18, 2006
Revised Manuscript: April 11, 2007
Manuscript Accepted: April 20, 2007
Published: July 6, 2007

Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics

Kwan Jeong, John J. Turek, and David D. Nolte, "Fourier-domain digital holographic optical coherence imaging of living tissue," Appl. Opt. 46, 4999-5008 (2007)

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