Abstract

To gain insight into brain tumor invasion, experiments are conducted on multicellular tumor spheroids grown in collagen gel. Typically, a radius of invasion is reported, which is obtained by human measurement. We present a simple, heuristic algorithm for automated invasive radii estimation (AIRE) that uses local fluctuations of the image intensity. We then derive an analytical expression relating the image graininess to the cell density for a model imaging system. The result agrees with the experiment up to a multiplicative constant and thus describes a novel method for estimating the cell density from bright-field images.

© 2007 Optical Society of America

Three-dimensional dynamics optical coherence tomography for tumor spheroid evaluation

Ibrahim Abd El-Sadek, Arata Miyazawa, Larina Tzu-Wei Shen, Shuichi Makita, Pradipta Mukherjee, Antonia Lichtenegger, Satoshi Matsusaka, and Yoshiaki Yasuno
Biomed. Opt. Express 12(11) 6844-6863 (2021)

Characterization and quantification of necrotic tissues and morphology in multicellular ovarian cancer tumor spheroids using optical coherence tomography

Feng Yan, Gokhan Gunay, Trisha I. Valerio, Chen Wang, Jayla A. Wilson, Majood S. Haddad, Maegan Watson, Michael O. Connell, Noah Davidson, Kar-Ming Fung, Handan Acar, and Qinggong Tang
Biomed. Opt. Express 12(6) 3352-3371 (2021)

Holographic optical coherence imaging of rat osteogenic sarcoma tumor spheroids

Ping Yu, Mirela Mustata, Leilei Peng, John J. Turek, Michael R. Melloch, Paul M. W. French, and David D. Nolte
Appl. Opt. 43(25) 4862-4873 (2004)

Intrinsic optical biomarkers associated with the invasive potential of tumor cells in engineered tissue models

Joanna Xylas, Addy Alt-Holland, Jonathan Garlick, Martin Hunter, and Irene Georgakoudi
Biomed. Opt. Express 1(5) 1387-1400 (2010)

Glioblastoma cells labeled by robust Raman tags for enhancing imaging contrast

Li-Ching Huang, Yung-Ching Chang, Yi-Syuan Wu, Wei-Lun Sun, Chan-Chuan Liu, Chun-I Sze, and Shiuan-Yeh Chen
Biomed. Opt. Express 9(5) 2142-2153 (2018)