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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 46, Iss. 22 — Aug. 1, 2007
  • pp: 5522–5528

Principal component analysis of fluorescence changes upon growth conditions and washing of Pseudomonas aeruginosa

Ekta Jhala, Craig Galilee, and Lou Reinisch  »View Author Affiliations

Applied Optics, Vol. 46, Issue 22, pp. 5522-5528 (2007)

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We have measured the autofluorescence from suspensions of Pseudomonas aeruginosa in the growth medium and after one, two, and three washes. The bacterium was grown in two different media, nutrient broth and King's B broth. The bacterium was harvested after 12, 24, and 48 h of growth. The fluorescence was measured with excitation every 10   nm from 200   nm to 600   nm . The fluorescence profiles were analyzed using principal component analysis. We found that most of the information is in the first three principal components. Stark differences in the value of the first principal component were noted between the samples in broth and those with one, two, or three washings. The second and third principal components noted differences between the samples washed once and those washed two or three times. There was no significant difference between samples washed two and three times. There are small differences noted between the samples grown in the two different broths, and no differences were noted among the samples harvested at different times.

© 2007 Optical Society of America

OCIS Codes
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence
(300.6540) Spectroscopy : Spectroscopy, ultraviolet

ToC Category:

Original Manuscript: September 11, 2006
Revised Manuscript: January 26, 2007
Manuscript Accepted: March 30, 2007
Published: July 31, 2007

Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics

Ekta Jhala, Craig Galilee, and Lou Reinisch, "Principal component analysis of fluorescence changes upon growth conditions and washing of Pseudomonas aeruginosa," Appl. Opt. 46, 5522-5528 (2007)

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