The main objective of this work was to investigate how the cell size and the presence of a cell wall influence the scattering properties of the green microalgae Chlamydomonas reinhardtii. The growth cycle of two strains, one with a cell wall and one without, was synchronized to be in the same growth phase. Measurements were conducted at two different phases of the growth cycle on both strains of the algae. It was found that the shape of the scattering phase function was very similar for both strains at both growth phases, but the regular strain with a cell wall scatters more strongly than the wall-less mutant. It was also found that the mutant strain has a stronger increase in scattering than the regular strain, as the algae grow, and that the scattering from the regular strain is more wavelength dependent than from the mutant strain.
© 2007 Optical Society of America
Original Manuscript: December 20, 2006
Revised Manuscript: March 29, 2007
Manuscript Accepted: May 1, 2007
Published: August 8, 2007
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics
Øyvind Svensen, Øyvind Frette, and Svein Rune Erga, "Scattering properties of microalgae: the effect of cell size and cell wall," Appl. Opt. 46, 5762-5769 (2007)