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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 46, Iss. 27 — Sep. 20, 2007
  • pp: 6815–6820

Optimization of image-forming optics for transmission optical projection tomography

Yi Wang and Ruikang K. Wang  »View Author Affiliations

Applied Optics, Vol. 46, Issue 27, pp. 6815-6820 (2007)

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A new optical system for transmission optical projection tomography (TOPT) is presented to reduce the divergence of the projection data from the true parallel projections. This is performed by introducing an iris at the back focus of the objective lens. The influence of the defocusing on TOPT is demonstrated by computational simulations and experiments. We compare the performances of the new and conventional TOPT systems in order to optimize the optical system for three-dimensional imaging of the embryos of small animals. The optimal imaging performance is given by the new system with numerical apertures between 0.007 and 0.014, with which the spatial resolution of 25 μm is achieved. The optimal configuration is validated by TOPT of a phantom sample and a fixed five-day chick embryo.

© 2007 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: January 29, 2007
Revised Manuscript: June 20, 2007
Manuscript Accepted: June 23, 2007
Published: September 19, 2007

Virtual Issues
Vol. 2, Iss. 10 Virtual Journal for Biomedical Optics

Yi Wang and Ruikang K. Wang, "Optimization of image-forming optics for transmission optical projection tomography," Appl. Opt. 46, 6815-6820 (2007)

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