Toward instrument-independent quantitative measurement of fluorescence intensity in fiber-optic spectrometer systems
Applied Optics, Vol. 46, Issue 29, pp. 7132-7140 (2007)
http://dx.doi.org/10.1364/AO.46.007132
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Abstract
Fluorescence has been widely used in biological research and clinical diagnosis. One challenge facing the rapid growth of fluorescence application is the inability to make comparable fluorescence intensity measurements during a long period of clinical study and across laboratories. We propose a method to implement system-independent fluorescence intensity calibration in fiber-optic fluorescence spectrometer systems. This method is based on a National Institute for Standards and Technology traceable standard light source for system spectral response calibration, and a fluorescence reference standard for fluorescence intensity calibration. Human skin in vivo and a fluorescence phantom made of fluorescent microspheres were measured with two different system configurations and at different probe-sample distances. Experimental results showed very good agreement with theory after system-independent fluorescence intensity calibration.
© 2007 Optical Society of America
OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
ToC Category:
Spectroscopy
History
Original Manuscript: January 17, 2007
Revised Manuscript: July 13, 2007
Manuscript Accepted: August 10, 2007
Published: October 4, 2007
Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics
Citation
Jianhua Zhao, Harvey Lui, David I. McLean, and Haishan Zeng, "Toward instrument-independent quantitative measurement of fluorescence intensity in fiber-optic spectrometer systems," Appl. Opt. 46, 7132-7140 (2007)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-46-29-7132
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