OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 46, Iss. 29 — Oct. 10, 2007
  • pp: 7329–7336

Using lasers to probe the transient light absorption by proteorhodopsin in marine bacterioplankton

Russell A. Desiderio, Samuel R. Laney, Ricardo M. Letelier, and Stephen J. Giovannoni  »View Author Affiliations


Applied Optics, Vol. 46, Issue 29, pp. 7329-7336 (2007)
http://dx.doi.org/10.1364/AO.46.007329


View Full Text Article

Enhanced HTML    Acrobat PDF (647 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We constructed an experimental apparatus that used lasers to provide the probe beams for measuring the transient absorption kinetics of bacterioplankton that contain proteorhodopsin, a microbial protein that binds retinal and is analogous to animal rhodopsin. With this approach we were able to observe photocycles characteristic of functioning retinylidene ion pumps. Using light from lasers instead of broadband sources as transmittance probe beams can be advantageous when examining optically dense, highly scattering samples such as concentrated microbial cultures. Such a laser-based approach may prove useful in shipboard studies for identifying proteorhodopsin in whole cell suspensions concentrated from seawater.

© 2007 Optical Society of America

OCIS Codes
(120.6200) Instrumentation, measurement, and metrology : Spectrometers and spectroscopic instrumentation
(300.6500) Spectroscopy : Spectroscopy, time-resolved

ToC Category:
Spectroscopy

History
Original Manuscript: May 7, 2007
Revised Manuscript: September 9, 2007
Manuscript Accepted: August 27, 2007
Published: October 9, 2007

Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Russell A. Desiderio, Samuel R. Laney, Ricardo M. Letelier, and Stephen J. Giovannoni, "Using lasers to probe the transient light absorption by proteorhodopsin in marine bacterioplankton," Appl. Opt. 46, 7329-7336 (2007)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-46-29-7329

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited