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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 46, Iss. 32 — Nov. 10, 2007
  • pp: 7836–7849

Common-path interferometric detection of protein monolayer on the BioCD

Xuefeng Wang, Ming Zhao, and D. D. Nolte  »View Author Affiliations

Applied Optics, Vol. 46, Issue 32, pp. 7836-7849 (2007)

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The bio-optical compact disk (BioCD) is an optical biosensor that performs common-path molecular interferometry of patterned proteins on a disk spinning at high speed. The common-path configuration makes it ultrastable and allows surface height precision below 10 pm. In this paper we show that two complementary interferometric quadrature conditions exist simultaneously that convert the modulus and phase of the reflection coefficient, modulated by protein patterns on the disk surface, into intensity modulation at the detector. In the far field they separate into spatially symmetric and antisymmetric intensity modulation in response to the local distribution of protein. The antisymmetric response is equivalent to differential phase-contrast detection, and the symmetric response is equivalent to in-line (IL) common-path interferometry. We measure the relative sensitivities of these orthogonal channels to printed protein patterns on disk structures that include thermal oxide on silicon and Bragg dielectric stacks. The scaling mass sensitivity of the IL channel on oxide on silicon was measured to be 0.17 pg / mm .

© 2007 Optical Society of America

OCIS Codes
(110.4190) Imaging systems : Multiple imaging
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(180.5810) Microscopy : Scanning microscopy
(310.6860) Thin films : Thin films, optical properties

ToC Category:

Original Manuscript: June 8, 2007
Revised Manuscript: August 29, 2007
Manuscript Accepted: August 31, 2007
Published: November 2, 2007

Virtual Issues
Vol. 2, Iss. 12 Virtual Journal for Biomedical Optics

Xuefeng Wang, Ming Zhao, and D. D. Nolte, "Common-path interferometric detection of protein monolayer on the BioCD," Appl. Opt. 46, 7836-7849 (2007)

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