OSA's Digital Library

Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 46, Iss. 33 — Nov. 20, 2007
  • pp: 8080–8088

Canine cancer screening via ultraviolet absorbance and fluorescence spectroscopy of serum proteins

Bryan D. Dickerson, Brian L. Geist, William B. Spillman, Jr., and John L. Robertson  »View Author Affiliations

Applied Optics, Vol. 46, Issue 33, pp. 8080-8088 (2007)

View Full Text Article

Enhanced HTML    Acrobat PDF (1221 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



A cost-effective optical cancer screening and monitoring technique was demonstrated in a pilot study of canine serum samples and was patented for commercialization. Compared to conventional blood chemistry analysis methods, more accurate estimations of the concentrations of albumin, globulins, and hemoglobin in serum were obtained by fitting the near UV absorbance and photoluminescence spectra of diluted serum as a linear combination of component reference spectra. Tracking these serum proteins over the course of treatment helped to monitor patient immune response to carcinoma and therapy. For cancer screening, 70% of dogs with clinical presentation of cancer displayed suppressed serum hemoglobin levels (below 20 mg / dL ) in combination with atypical serum protein compositions, that is, albumin levels outside of a safe range (from 4 to 8 g / dL ) and globulin levels above or below a more normal range (from 1.7 to 3.7 g / dL ). Of the dogs that met these criteria, only 20% were given a false positive label by this cancer screening test.

© 2007 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.1610) Medical optics and biotechnology : Clinical applications
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: March 22, 2007
Revised Manuscript: September 30, 2007
Manuscript Accepted: October 5, 2007
Published: November 19, 2007

Virtual Issues
Vol. 2, Iss. 12 Virtual Journal for Biomedical Optics

Bryan D. Dickerson, Brian L. Geist, William B. Spillman, Jr., and John L. Robertson, "Canine cancer screening via ultraviolet absorbance and fluorescence spectroscopy of serum proteins," Appl. Opt. 46, 8080-8088 (2007)

Sort:  Year  |  Journal  |  Reset  


  1. R. S. Cotran, V. Kumar, and T. Collins, eds., Robbins Pathological Basis of Disease (Saunders, 1999), pp. 260-327.
  2. R. Richards-Kortum, R. Drezek, K. Basen-Engquist, S. B. Cantor, U. Utzinger, C. Brookner, and M. Follen, "Cervical dysplasia diagnosis with fluorescence spectroscopy," in Handbook of Biomedical Fluorescence, M. A. Mycek and B. W. Poque, eds. (Marcel Dekker, 2003), pp. 265-314.
  3. G. Wagnières, A. McWilliams, and S. Lam, "Lung cancer imaging with fluorescence endoscopy," in Handbook of Biomedical Fluorescence, M. A. Mycek and B. W. Poque, eds. (Marcel Dekker, 2003), pp. 361-396.
  4. E. P. Diamandis, "How are we going to discover new cancer biomarkers? A proteomic approach for bladder cancer," Clin. Chem. 50, 793-795 (2004). [CrossRef] [PubMed]
  5. S. C. Gad, ed., Drug Discovery Handbook (Wiley-Interscience, 2005), pp. 81-83.
  6. K. P. H. Pritzker, "Cancer biomarkers: Easier said than done," Clin. Chem. 48, 1147-1150 (2002). [PubMed]
  7. R. Aebersold and M. Mann, "Mass spectroscopy-based proteomics," Nature (London) 422, 198-207 (2003). [CrossRef] [PubMed]
  8. J. Li, Z. Zhang, J. Rosenzweig, Y. Y. Wang, and D. W. Chan, "Proteomics and bioinformatics approaches for identification of serum biomarkers to detect breast cancer," Clin. Chem. 48, 1296-1304 (2002). [PubMed]
  9. A. Statnikov, C. Aliferis, I. Tsamardinos, D. Hardin, and S. Levy, "A comprehensive evaluation of multicategory classification methods for microarray gene expression cancer diagnosis," Bioinformatics 21, 631-643 (2005). [CrossRef]
  10. A. A. Pineda, ed., Selective Plasma Component Removal (Futura, 1984), p. 156.
  11. B. F. Feldman, J. G. Zinkl, and N. C. Jain, eds., Schalm's Veterinary Hematology, 5th ed. (Lippincott Williams & Wilkins, 2000), pp. 565-570 and 899-903.
  12. M. R. Hubmann, M. J. P. Leiner, and R. J. Schaur, "Ultraviolet fluorescence of human sera: I. Sources of characteristic differences in ultraviolet fluorescence spectra from sera of normal and cancer-bearing patients," Clin. Chem. 36, 1880-1883 (1990). [PubMed]
  13. S. Welle, Human Protein Metabolism (Springer, 1999), pp. 204-204.
  14. R. J. Henry, D. C. Cannon, and J. W. Winkelman, eds., Clinical Chemistry Principles and Techniques, 2nd ed. (Harper & Row, 1974), pp. 449, 1071-1072, 1117, and 1239.
  15. M. Kondo, N. Hirota, T. Takaoka, and M. Kajiwara, "Heme biosynthetic enzyme activities and porphyrin accumulation in normal liver and hepatoma cell lines of rat," Cell. Biol. Toxins 9, 95-105 (1993). [CrossRef]
  16. R. Beri and R. Chandra, "Chemistry and biology of heme: effect of metal salts, organometals, and metalloproteins on heme synthesis and catabolism, with special reference to clinical implications and interactions with cytochrome P-450," Drug Metab. Rev. 25, 49-152 (1993). [CrossRef] [PubMed]
  17. B. R. Munson and R. J. Fiel, "A review: biochemical alterations associated with mouse spleen cells infected with Friend virus," J. Med. 4, 354-370 (1973). [PubMed]
  18. T. S. Mang, C. McGinnis, C. Liebow, U. O. Nseyo, D. H. Cream, and T. J. Dougherty, "Fluorescence detection of tumors," Cancer 71, 269-276 (1993). [CrossRef] [PubMed]
  19. D. M. Harris and J. Werkhaven, "Endogenous porphyrin fluorescence in tumors," Lasers Surg. Med. 7, 467-472 (1987). [CrossRef] [PubMed]
  20. P. Jichlinski, M. Forrer, J. Mizeret, T. Glanzmann, D. Braichotte, G. Wagnières, G. Zimmer, L. Gulillou, F. Schmidlin, P. Graber, H. van den Bergh, and H. J. Leisinger, "Clinical evaluation of a method for detecting superficial transitional cell carcinoma of the bladder by light-induced fluorescence of protoporphyrin IX following topical application of 5-aminolevulinic acid," Lasers Surg. Med. 20, 402-408 (1997). [CrossRef] [PubMed]
  21. B. Leca-Bouvier and L. J. Blum, "Biosensors for protein detection: a review," Anal. Lett. 38, 1491-1517 (2005). [CrossRef]
  22. C. V. Sapan, R. L. Lundblad, and N. C. Price, "Colorimetric protein assay techniques," Biotechnol. Appl. Biochem. 29, 99-108 (1999). [PubMed]
  23. M. Weiss, C. L. Loprinzi, E. T. Creagan, R. J. Dalton, P. Novotny, and J. R. O'Fallon, "Utility of follow-up tests for detecting recurrent disease in patients with malignant melanomas," J. Am. Med. Assoc. 274, 1703-1705 (1995). [CrossRef]
  24. L. Pena, M. D. Perez-Alenza, A. Rodriguez-Bertos, and A. Nieto, "Canine inflammatory mammary carcinoma: histopathology, immunohistochemistry and clinical implications of 21 cases," Breast Cancer Res. Treat. 78, 141-148 (2003). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited