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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 46, Iss. 4 — Feb. 1, 2007
  • pp: 527–532

Particle sizing with a fast polar nephelometer

Jean-Luc Castagner and Irving J. Bigio  »View Author Affiliations


Applied Optics, Vol. 46, Issue 4, pp. 527-532 (2007)
http://dx.doi.org/10.1364/AO.46.000527


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Abstract

We reported previously the design of a polar nephelometer that uses a rotational confocal imaging setup to enable fast scanning of the scattering phase function within a field of view of 55°. The full dynamic range of the detection system can be used by increasing the signal- to-noise ratio by means of averaging successive scans. The calibration of the angular response of the instrument is achieved by obtaining the transfer function of the optical detection system using Rayleigh scatterers. Accurate particle sizing of individual polystyrene spheres (ranging from 1.5 to 9   μm in diameter) in aqueous suspension is achieved by maximizing a correlation coefficient between precalculated tables of Mie phase functions and data obtained from the polar nephelometer. Good correlation is achieved between experimental and theoretical data, proving the functioning of the instrument as a fast and convenient particle sizer.

© 2007 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(290.4020) Scattering : Mie theory
(290.5820) Scattering : Scattering measurements
(290.5850) Scattering : Scattering, particles

ToC Category:
Scattering

History
Original Manuscript: June 19, 2006
Revised Manuscript: September 8, 2006
Manuscript Accepted: September 13, 2006

Virtual Issues
Vol. 2, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Jean-Luc Castagner and Irving J. Bigio, "Particle sizing with a fast polar nephelometer," Appl. Opt. 46, 527-532 (2007)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-46-4-527

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