Quantitative structured-illumination phase microscopy
Applied Optics, Vol. 47, Issue 1, pp. 15-24 (2008)
http://dx.doi.org/10.1364/AO.47.000015
Enhanced HTML 
Acrobat PDF (1154 KB)
Abstract
We introduce a quantitative phase imaging method for homogeneous objects with a bright field transmission microscope by using an amplitude mask and a digitalprocessing algorithm. A known amplitude pattern is imaged on the sample plane containing a thick phase object by placing an amplitude mask in the field diaphragm of the microscope. The phase object distorts the amplitude pattern according to its optical path length (OPL) profile, and the distorted pattern is recorded in a CCD detector. A digitalprocessing algorithm then estimates the object's quantitative OPL profile based on a closed form analytical solution, which is derived using a ray optics model for objects with small OPL gradients.
© 2008 Optical Society of America
OCIS Codes
(110.2960) Imaging systems : Image analysis
(120.3940) Instrumentation, measurement, and metrology : Metrology
(180.0180) Microscopy : Microscopy
(350.5030) Other areas of optics : Phase
(110.1758) Imaging systems : Computational imaging
ToC Category:
Microscopy
History
Original Manuscript: July 2, 2007
Revised Manuscript: November 2, 2007
Manuscript Accepted: November 11, 2007
Published: December 20, 2007
Virtual Issues
Vol. 3, Iss. 2 Virtual Journal for Biomedical Optics
Citation
Sri Rama Prasanna Pavani, Ariel R. Libertun, Sharon V. King, and Carol J. Cogswell, "Quantitative structured-illumination phase microscopy," Appl. Opt. 47, 15-24 (2008)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-47-1-15
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Phase-shifting polarization interferometry for microstructure linewidth measurement (OL)
- Imaging Performance of Telescope Mirrors for Far-Ultraviolet Astronomy (AO)
- Defocused transfer function for a partially coherent microscope and application to phase retrieval (JOSAA)
- Imaging analysis of digital holography (OE)
- Direct extraction of the mean particle size from a digital hologram (AO)
Related Conference Papers
- Structured-Illumination Quantitative Phase Microscopy
- Quantitative Phase Estimation with a Bright Field Microscope
- Optical Artifacts </b><b>in Digital Video Microscopy
- 3-D Fluorescent Particle Tracking with Nanometer Scale Accuracies Using a Double-Helix Point Spread Function
- Single Molecule Imaging of Axonal Transport in Live Neurons
« Previous Article | Next Article »
OSA is a member of 