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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 47, Iss. 10 — Apr. 1, 2008
  • pp: 1608–1616

Multispectral fluorescence lifetime imaging of feces-contaminated apples by time-resolved laser-induced fluorescence imaging system with tunable excitation wavelengths

Moon S. Kim, Byoung-Kwan Cho, Alan M. Lefcourt, Yud-Ren Chen, and Sukwon Kang  »View Author Affiliations


Applied Optics, Vol. 47, Issue 10, pp. 1608-1616 (2008)
http://dx.doi.org/10.1364/AO.47.001608


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Abstract

We recently developed a time-resolved multispectral laser-induced fluorescence (LIF) imaging system capable of tunable wavelengths in the visible region for sample excitation and nanosecond-scale characterizations of fluorescence responses (lifetime imaging). Time-dependent fluorescence decay characteristics and fluorescence lifetime imaging of apples artificially contaminated with a range of diluted cow feces were investigated at 670 and 685 nm emission bands obtained by 418, 530, and 630 nm excitations. The results demonstrated that a 670 nm emission with a 418 nm excitation provided the greatest difference in time-dependent fluorescence responses between the apples and feces-treated spots. The versatilities of the time-resolved LIF imaging system, including fluorescence lifetime imaging of a relatively large biological object in a multispectral excitation-emission wavelength domain, were demonstrated.

© 2008 Optical Society of America

OCIS Codes
(110.2970) Imaging systems : Image detection systems
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(300.2530) Spectroscopy : Fluorescence, laser-induced
(280.1415) Remote sensing and sensors : Biological sensing and sensors
(110.4234) Imaging systems : Multispectral and hyperspectral imaging

ToC Category:
Imaging Systems

History
Original Manuscript: August 31, 2007
Revised Manuscript: January 15, 2008
Manuscript Accepted: January 29, 2008
Published: March 31, 2008

Virtual Issues
Vol. 3, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Moon S. Kim, Byoung-Kwan Cho, Alan M. Lefcourt, Yud-Ren Chen, and Sukwon Kang, "Multispectral fluorescence lifetime imaging of feces-contaminated apples by time-resolved laser-induced fluorescence imaging system with tunable excitation wavelengths," Appl. Opt. 47, 1608-1616 (2008)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-47-10-1608


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