OSA's Digital Library

Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 47, Iss. 11 — Apr. 10, 2008
  • pp: 1734–1739

Estimation of optical properties of turbid media: experimental comparison of spatially and temporally resolved reflectance methods

Julie Falconet, Aurélie Laidevant, Raphaël Sablong, Anabela da Silva, Michel Berger, Franck Jaillon, Emmanuel Perrin, Jean-Marc Dinten, and Hervé Saint-Jalmes  »View Author Affiliations

Applied Optics, Vol. 47, Issue 11, pp. 1734-1739 (2008)

View Full Text Article

Enhanced HTML    Acrobat PDF (1321 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We compare two methods for the optical characterization of turbid media. The estimates of the absorption and reduced scattering coefficients ( μ a and μ s ) by a spatially resolved method and a time-resolved method are performed on tissue-like phantoms. Aqueous suspension of microspheres and Intralipid are used as scattering media with the addition of ink as an absorber. μ s is first measured on weakly absorbing media. The robustness of these measurements is then tested with respect to a variation of μ a . The spatially resolved method gave more accurate estimates for μ s whereas the time-resolved method gave better results for μ a estimates.

© 2008 Optical Society of America

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5280) Medical optics and biotechnology : Photon migration
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(170.7050) Medical optics and biotechnology : Turbid media

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: June 18, 2007
Revised Manuscript: February 7, 2008
Manuscript Accepted: February 12, 2008
Published: April 7, 2008

Virtual Issues
Vol. 3, Iss. 5 Virtual Journal for Biomedical Optics

Julie Falconet, Aurélie Laidevant, Raphaël Sablong, Anabela da Silva, Michel Berger, Franck Jaillon, Emmanuel Perrin, Jean-Marc Dinten, and Hervé Saint-Jalmes, "Estimation of optical properties of turbid media: experimental comparison of spatially and temporally resolved reflectance methods," Appl. Opt. 47, 1734-1739 (2008)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. P. van der Zee, “Methods for measuring the optical properties of tissue samples in the visible and near-infrared wavelength range,” in Medical Optical Tomography: Functional Imaging and Monitoring, G.J.Mueller, B.Chance, R.R.Alfano, S.R.Arridge, J.Beuthan, E.Gratton, M.F.Kaschke, B.R.Masters, S.Svanberg, and P.van der Zee, eds. (SPIE, 1993), pp. 166-192.
  2. A. Kienle, M. S. Patterson, N. Dögnitz, R. Bays, G. Wagnières, and H. van den Bergh, “Noninvasive determination of the optical properties of two-layered turbid media,” Appl. Opt. 37, 779-791 (1998). [CrossRef]
  3. J. R. Mourant, J. P. Freyer, A. H. Hielscher, A. A. Eick, A. Shen, and T. M. Johnson, “Mechanisms of light scattering from biological cells relevant to noninvasive optical-tissue diagnostics,” Appl. Opt. 37, 3586-3593 (1998). [CrossRef]
  4. L. Voisin-Gobin, L. Blanchot, and H. Saint-Jalmes, “Integrating the digitized backscattered image to measure absorption and reduced scattering coefficients in vivo,” Appl. Opt. 38, 4217-4227 (1999). [CrossRef]
  5. J. Swartling, J. S. Dam, and S. Andersson-Engels, “Comparison of spatially and temporally resolved diffuse-reflectance measurement systems for determination of biomedical optical properties,” Appl. Opt. 42, 4612-4620 (2003). [CrossRef] [PubMed]
  6. R. Cubeddu, M. Musolino, A. Pifferi, P. Taroni, and G. Valentini, “Time-resolved reflectance: a systematic study for application to the optical characterization of tissues,” IEEE J. Quantum Electron. 30, 2421-2430 (1994). [CrossRef]
  7. S. L. Jacques, “Time-resolved reflectance spectroscopy in turbid tissues,” IEEE Trans. Biomed. Eng. 36, 1155-1161 (1989). [CrossRef] [PubMed]
  8. S. Andersson-Engels, R. Berg, S. Svanberg, and O. Jarlman, “Time-resolved transillumination for medical diagnostics,” Opt. Lett. 15, 1179-1181 (1990). [CrossRef] [PubMed]
  9. D. T. Delpy, M. Cope, P. van der Zee, S. Arridge, S. Wray, and J. Wyatt, “Estimation of optical pathlength through tissue from direct time of flight measurement,” Phys. Med. Biol. 33, 1433-1442 (1988). [CrossRef] [PubMed]
  10. W. Becker, A. Bergmann, G. Biscotti, and A. Rück, “Advanced time-correlated single photon counting technique,” Proc. SPIE , 5340104-112 (2004). [CrossRef]
  11. M. Patterson, B. Chance, and B. Wilson, “Time resolved reflectance and transmittance for the noninvasive measurement of tissue optical properties,” Appl. Opt. 28, 2331-2336 (1989). [CrossRef] [PubMed]
  12. A. Laidevant, A. da Silva, M. Berger, and J.-M. Dinten, “Effects of the surface boundary on the determination of the optical properties of a turbid medium with time-resolved reflectance,” Appl. Opt. 45, 4756-4764 (2006). [CrossRef] [PubMed]
  13. X. Smith, R. C. Baker, and K. S. Baker, “Optical properties of the clearest natural waters (200800 nm),” Appl. Opt. 20, 177-184 (1981). [CrossRef] [PubMed]
  14. H. van Staveren, C. Moes, J. van Marle, S. Prahl, and M. van Gemert, “Light scattering in Intralipid-10% in the wavelength range of 4001100 nm,” Appl. Opt. 30, 4507-4514 (1991). [CrossRef] [PubMed]
  15. F. Jaillon, “Caractérisation optique des milieux diffusants: simulation Monte Carlo et mesures en rétrodiffusion polarisée,” Ph.D. dissertation (U. Claude Bernard Lyon 1, 2003).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited