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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 47, Iss. 12 — Apr. 20, 2008
  • pp: 2011–2016

Diffuse optical tomography guided quantitative fluorescence molecular tomography

Yiyong Tan and Huabei Jiang  »View Author Affiliations


Applied Optics, Vol. 47, Issue 12, pp. 2011-2016 (2008)
http://dx.doi.org/10.1364/AO.47.002011


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Abstract

We describe a method that combines fluorescence molecular tomography (FMT) with diffuse optical tomography (DOT), which allows us to study the impact of heterogeneous optical property distribution on FMT, an issue that has not been systemically studied. Both numerical simulations and phantom experiments were performed based on our finite-element reconstruction algorithms. The experiments were conducted using a noncontact optical fiber free, multiangle transmission system. In both the simulations and experiments, a fluorescent target was embedded in an optically heterogeneous background medium. The simulation results clearly suggest the necessity of considering the absorption coefficient ( μ a ) and reduced scattering coefficient ( μ s ) distributions for quantitatively accurate FMT, especially in terms of the accuracy of reconstructed fluorophore absorption coefficient ( μ a x m ). Subsequent phantom experiments with an indocyanine green (ICG)-containing target confirm the simulation findings. In addition, we performed a series of phantom experiments with low ICG concentration (0.1, 0.2, 0.4, 0.6 and 1.0 μM ) in the target to systematically evaluate the quantitative accuracy of our FMT approach. The results indicate that, with the knowledge of optical property distribution, the accuracy of the recovered fluorophore concentration is improved significantly over that without such a priori information. In particular absolute value of μ a x m from our DOT guided FMT are quantitatively consistent with that obtained using spectroscopic methods.

© 2008 Optical Society of America

OCIS Codes
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

ToC Category:
Imaging Systems

History
Original Manuscript: August 22, 2007
Revised Manuscript: February 22, 2008
Manuscript Accepted: March 14, 2008
Published: April 11, 2008

Virtual Issues
Vol. 3, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Yiyong Tan and Huabei Jiang, "Diffuse optical tomography guided quantitative fluorescence molecular tomography," Appl. Opt. 47, 2011-2016 (2008)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-47-12-2011

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