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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 47, Iss. 12 — Apr. 20, 2008
  • pp: 2028–2034

Simultaneous detection of molecular oxygen and water vapor in the tissue optical window using tunable diode laser spectroscopy

Linda Persson, Märta Lewander, Mats Andersson, Katarina Svanberg, and Sune Svanberg  »View Author Affiliations

Applied Optics, Vol. 47, Issue 12, pp. 2028-2034 (2008)

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We report on a dual-diode laser spectroscopic system for simultaneous detection of two gases. The technique is demonstrated by performing gas measurements on absorbing samples such as an air distance, and on absorbing and scattering porous samples such as human tissue. In the latter it is possible to derive the concentration of one gas by normalizing to a second gas of known concentration. This is possible if the scattering and absorption of the bulk material is equal or similar for the two wavelengths used, resulting in a common effective pathlength. Two pigtailed diode lasers are operated in a wavelength modulation scheme to detect molecular oxygen 760 nm and water vapor 935 nm within the tissue optical window ( 600 nm to 1.3 μm ). Different modulation frequencies are used to distinguish between the two wavelengths. No crosstalk can be observed between the gas contents measured in the two gas channels. The system is made compact by using a computer board and performing software-based lock-in detection. The noise floor obtained corresponds to an absorption fraction of approximately 6 × 10 - 5 for both oxygen and water vapor, yielding a minimum detection limit of 2 mm for both gases in ambient air. The power of the technique is illustrated by the preliminary results of a clinical trial, nonintrusively investigating gas in human sinuses.

© 2008 Optical Society of America

OCIS Codes
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.7050) Medical optics and biotechnology : Turbid media
(300.1030) Spectroscopy : Absorption
(300.6260) Spectroscopy : Spectroscopy, diode lasers

ToC Category:

Original Manuscript: September 4, 2007
Revised Manuscript: February 8, 2008
Manuscript Accepted: February 28, 2008
Published: April 14, 2008

Virtual Issues
Vol. 3, Iss. 5 Virtual Journal for Biomedical Optics

Linda Persson, Märta Lewander, Mats Andersson, Katarina Svanberg, and Sune Svanberg, "Simultaneous detection of molecular oxygen and water vapor in the tissue optical window using tunable diode laser spectroscopy," Appl. Opt. 47, 2028-2034 (2008)

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  1. J. Faist, F. Capasso, D. Sivco, C. Sirtori, A. Hutchinson, and A. Cho, “Quantum cascade laser,” Science 264, 553-556 (1994). [CrossRef] [PubMed]
  2. G. Wysocki, M. McCurdu, S. So, D. Weidmann, C. Roller, R. Curl, and F. Tittel, “Pulsed quantum-cascade laser-based sensor for trace-gas detection of carbonyl sulfide,” Appl. Opt. 43, 6040-6046 (2004). [CrossRef] [PubMed]
  3. M. Silva, D. Sonnenfroh, D. Rosen, M. Allen, and A. O'Keefe, “Integrated cavity output spectroscopy measurements of nitric oxide levels in breath with a pulsed room-temperature quantum cascade laser,” Appl. Phys. B 81, 705-710 (2005). [CrossRef]
  4. J. Parrish, “New concepts in therapeutic photomedicine: photochemistry, optical targeting and the therapeutic window,” J. Investigative Dermatol. 77, 45-50 (1981). [CrossRef]
  5. J. Boulnois, “Photophysical processes in recent medical laser developments: a review,” Lasers Med. Sci. 1, 47-66 (1986). [CrossRef]
  6. G. Somesfalean, Z. Zhang, M. Sjöholm, and S. Svanberg, “All-diode-laser ultraviolet absorption spectroscopy for sulfur dioxide detection,” Appl. Phys. B 80, 1021-1025 (2005). [CrossRef]
  7. U. Gustafsson, J. Sandsten, and S. Svanberg, “Simultaneous detection of methane, oxygen and water vapour utilizing near-infrared diode lasers in conjunction with difference frequency generation,” Appl. Phys. B 71, 853-857 (2000).
  8. J. Reid and D. Labrie, “Second-harmonic detection with tunable diode lasers--comparison of experiment and theory,” Appl. Phys. B 26, 203-210 (1981). [CrossRef]
  9. M. Andersson, L. Persson, T. Svensson, and S. Svanberg, “Flexible lock-in detection system based on synchronized computer plug-in boards applied in sensitive gas spectroscopy,” Rev. Sci. Instrum. 78, 113107 (2007). [CrossRef] [PubMed]
  10. L. Persson, F. Andersson, M. Andersson, and S. Svanberg, “Approach to optical interference fringes reduction in diode laser absorption spectroscopy,” Appl. Phys. B 87, 523-530 (2007). [CrossRef]
  11. L. Persson, M. Andersson, T. Svensson, M. Cassel-Engquist, K. Svanberg, and S. Svanberg, “Non-intrusive optical study of gas and its exchange in human maxillary sinuses,” Proc. SPIE 6628, 662804-1-7 (2007).
  12. L. Persson, M. Andersson, M. Cassel-Engquist, K. Svanberg, and S. Svanberg, “Gas monitoring in human sinuses using tunable diode laser spectroscopy,” J Biomed. Opt. 12, 054001(2007). [CrossRef] [PubMed]

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