OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 47, Iss. 12 — Apr. 20, 2008
  • pp: 2070–2075

Stabilizing method for reflection interference contrast microscopy

Kipom Kim and Omar A. Saleh  »View Author Affiliations


Applied Optics, Vol. 47, Issue 12, pp. 2070-2075 (2008)
http://dx.doi.org/10.1364/AO.47.002070


View Full Text Article

Enhanced HTML    Acrobat PDF (2731 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Reflection interference contrast microscopy (RICM) is a technique for measuring the shape and position of microscopic objects in solution; it has many biological and biophysical applications. Use of RICM for long-time acquisitions requires minimizing defocusing effects that are due to thermal and mechanical drift. We present a simple stabilizing method that accomplishes this using an image-analysis-based linear focus function to establish feedback control of the focal position. While implementing this routine, we used RICM for independent measurement of the apparent fluctuation in the vertical position of an immobilized bead: the measured height had a standard deviation of 0.12 nm during a 45 min acquisition while under feedback control, demonstrating the high stability achievable with our approach.

© 2008 Optical Society of America

OCIS Codes
(120.2650) Instrumentation, measurement, and metrology : Fringe analysis
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.3170) Microscopy : Interference microscopy

ToC Category:
Instrumentation, Measurement, and Metrology

History
Original Manuscript: August 16, 2007
Revised Manuscript: February 19, 2008
Manuscript Accepted: February 19, 2008
Published: April 16, 2008

Virtual Issues
Vol. 3, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Kipom Kim and Omar A. Saleh, "Stabilizing method for reflection interference contrast microscopy," Appl. Opt. 47, 2070-2075 (2008)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-47-12-2070


Sort:  Year  |  Journal  |  Reset  

References

  1. A. S. G. Curtis, "The mechanism of adhesion of cells to glass: a study by interference reflection microscopy," J. Cell Biol. 20, 199-215 (1964). [CrossRef] [PubMed]
  2. J. Rädler and E. Sackmann, "Imaging optical thicknesses and separation distances of phospholipid vesicles at solid surfaces," J. Phys. (Paris) II 3, 727-748 (1993).
  3. Z. Guttenberg, A. R. Bausch, B. Hu, R. Bruinsma, L. Moroder, and E. Sackmann, "Measuring ligand-receptor unbinding forces with magnetic beads: molecular leverage," Langmuir 16, 8984-8993 (2000). [CrossRef]
  4. V. Heinrich, K. Ritchie, N. Mohandas, and E. Evans, "Elastic thickness compressibility of the red cell membrane," Biophys. J. 81, 1452-1463 (2001). [CrossRef] [PubMed]
  5. A. Zidovska and E. Sackmann, "Brownian motion of nucleated cell envelopes impedes adhesion," Phys. Rev. Lett. 96, 048103 (2006). [CrossRef] [PubMed]
  6. D. Cuvelier, M. Théry, Y.-S. Chu, S. Dufour, J.-P. Thiéry, M. Bornens, P. Nassoy, and L. Mahadevan, "The universal dynamics of cell spreading," Curr. Biol. 17, 694-699 (2007). [CrossRef] [PubMed]
  7. J. Rädler and E. Sackmann, "On the measurement of weak repulsive and frictional colloidal forces by reflection interference contrast microscopy," Langmuir 8, 848-853 (1992). [CrossRef]
  8. G. Wiegand, K. R. Neumaier, and E. Sackmann, "Microinterferometry: three-dimensional reconstruction of surface microtopography for thin-film and wetting studies by reflection interference contrast microscopy (RICM)," Appl. Opt. 37, 6892-6905 (1998). [CrossRef]
  9. N. G. Clack and J. T. Groves, "Many-particle tracking with nanometer resolution in three dimensions by reflection interference contrast microscopy," Langmuir 21, 6430-6435 (2005). [CrossRef] [PubMed]
  10. S. J. Gokhale, J. L. Plawsky, and P. C. Wayner, "Spreading, evaporation, and contact line dynamics of surfactant-laden microdrops," Langmuir 21, 8188-8197 (2005). [CrossRef] [PubMed]
  11. M. Kühner and E. Sackmann, "Ultrathin hydrated dextran films grafted on glass: preparation and characterization of structural, viscous, and elastic properties by quantitative microinterferometry," Langmuir 12, 4866-4876 (1996). [CrossRef]
  12. J.-M. Geusebroek, F. Cornelissen, A. W. M. Smeulders, and H. Geerts, "Robust autofocusing in microscopy," Cytometry 39, 1-9 (2000). [CrossRef] [PubMed]
  13. Y. Sun, S. Duthaler, and B. J. Nelson, "Autofocusing in computer microscopy: selecting the optimal focus algorithm," Microsc. Res. Tech. 65, 139-149 (2004). [CrossRef] [PubMed]
  14. M. Capitanio, R. Cicchi, and F. S. Pavone, "Position control and optical manipulation for nanotechnology applications," Eur. Phys. J. B 46, 1-8 (2005). [CrossRef]
  15. H. Oku, M. Ishikawa, Theodorus, and K. Hashimoto, "High-speed autofocusing of a cell using diffraction pattern," Opt. Express 14, 3952-3960 (2006). [CrossRef]
  16. A. R. Carter, G. M. King, T. A. Ulrich, W. Halsey, D. Alchenberger, and T. T. Perkins, "Stabilization of an optical microscope to 0.1 nm in three dimensions," Appl. Opt. 46, 421-427(2007). [CrossRef] [PubMed]
  17. W. J. Greenleaf, M. T. Woodside, and S. M. Block, "High-resolution, single-molecule measurements of biomolecular motion," Annu. Rev. Biophys. Biomol. Struct. 36, 171-190(2007). [CrossRef] [PubMed]
  18. T. Strick, J.-F. Allemand, V. Croquette, and D. Bensimon, "Twisting and stretching single DNA molecules," Prog. Biophys. Mol. Biol. 74, 115-140 (2000). [CrossRef] [PubMed]
  19. C. Bustamante, Z. Bryant, and S. B. Smith, "Ten years of tension: single-molecule DNA mechanics," Nature 421, 423-427(2003). [CrossRef] [PubMed]
  20. T. Lionnet, A. Dawid, S. Bigot, F.-X. Barre, O. A. Saleh, F. Heslot, J.-F. Allemand, D. Bensimon, and V. Croquette, "DNA mechanics as a tool to probe helicase and translocase activity," Nucleic Acids Res. 34, 4232-4244 (2006). [CrossRef] [PubMed]
  21. E. Krotkov, "Focusing," Int. J. Comput. Vision 1, 223-237(1988). [CrossRef]
  22. E. Sackmann and R. F. Bruinsma, "Cell adhesion as wetting transition?" ChemPhysChem. 3, 262-269 (2002). [CrossRef] [PubMed]
  23. P. C. Nelson, C. Zurla, D. Brogioli, J. F. Beausang, L. Finzi, and D. Dunlap, "Tethered particle motion as a diagnostic of DNA tether length," J. Phys. Chem. B 110, 17260-17267(2006). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited