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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 47, Iss. 15 — May. 20, 2008
  • pp: 2779–2789

Combined fluorescent and interferometric detection of protein on a BioCD

Xuefeng Wang, Ming Zhao, and D. D. Nolte  »View Author Affiliations


Applied Optics, Vol. 47, Issue 15, pp. 2779-2789 (2008)
http://dx.doi.org/10.1364/AO.47.002779


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Abstract

We perform simultaneous interferometric and fluorescent detection of molecular protein layers on a BioCD. The 488 nm excitation wavelength of fluorescein also provides the interferometric detection channel that operates in a common-path in-line configuration in the condition of phase quadrature set by a thermal oxide on silicon. The simultaneous acquisition of both channels enables a direct correlation between bound mass and fluorescent surface density, which we compare in forward- and reverse-phase immunoassays. Scaling mass sensitivities for immunoassays measured in the interferometric and fluorescent channels are 15 pg/mm and 1.5 pg/mm, respectively, when applied to gel-printed periodic antibody patterns detected in the frequency domain from the spinning disc. These sensitivities are limited by the inhomogeneities of the print. While fluorescence is subject to bleaching, the interferometry signal is robust under long-term laser illumination.

© 2008 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(310.6860) Thin films : Thin films, optical properties

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 20, 2008
Manuscript Accepted: March 28, 2008
Published: May 13, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Xuefeng Wang, Ming Zhao, and D. D. Nolte, "Combined fluorescent and interferometric detection of protein on a BioCD," Appl. Opt. 47, 2779-2789 (2008)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-47-15-2779

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