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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 47, Iss. 18 — Jun. 20, 2008
  • pp: 3275–3288

Protein reactions with surface-bound molecular targets detected by oblique-incidence reflectivity difference microscopes

J. P. Landry, Y. S. Sun, X. W. Guo, and X. D. Zhu  »View Author Affiliations

Applied Optics, Vol. 47, Issue 18, pp. 3275-3288 (2008)

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We applied oblique-incidence reflectivity difference microscopes (a form of polarization-modulated nulling ellipsometry) to detection of biomolecular microarrays without external labeling in a study of protein reactions with surface-immobilized targets. We show that the optical reflectivity difference signals can be quantitatively related to changes in surface mass density of molecular layers as a result of the reactions. Our experimental results demonstrate the feasibility of using oblique-incidence reflectivity difference microscopes for high-throughput proteomics research such as screening unlabeled protein probes against libraries of surface-immobilized small molecules.

© 2008 Optical Society of America

OCIS Codes
(170.5810) Medical optics and biotechnology : Scanning microscopy
(240.2130) Optics at surfaces : Ellipsometry and polarimetry

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: February 4, 2008
Revised Manuscript: May 7, 2008
Manuscript Accepted: May 9, 2008
Published: June 11, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

J. P. Landry, Y. S. Sun, X. W. Guo, and X. D. Zhu, "Protein reactions with surface-bound molecular targets detected by oblique-incidence reflectivity difference microscopes," Appl. Opt. 47, 3275-3288 (2008)

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