OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 47, Iss. 18 — Jun. 20, 2008
  • pp: 3275–3288

Protein reactions with surface-bound molecular targets detected by oblique-incidence reflectivity difference microscopes

J. P. Landry, Y. S. Sun, X. W. Guo, and X. D. Zhu  »View Author Affiliations


Applied Optics, Vol. 47, Issue 18, pp. 3275-3288 (2008)
http://dx.doi.org/10.1364/AO.47.003275


View Full Text Article

Enhanced HTML    Acrobat PDF (13336 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We applied oblique-incidence reflectivity difference microscopes (a form of polarization-modulated nulling ellipsometry) to detection of biomolecular microarrays without external labeling in a study of protein reactions with surface-immobilized targets. We show that the optical reflectivity difference signals can be quantitatively related to changes in surface mass density of molecular layers as a result of the reactions. Our experimental results demonstrate the feasibility of using oblique-incidence reflectivity difference microscopes for high-throughput proteomics research such as screening unlabeled protein probes against libraries of surface-immobilized small molecules.

© 2008 Optical Society of America

OCIS Codes
(170.5810) Medical optics and biotechnology : Scanning microscopy
(240.2130) Optics at surfaces : Ellipsometry and polarimetry

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 4, 2008
Revised Manuscript: May 7, 2008
Manuscript Accepted: May 9, 2008
Published: June 11, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

Citation
J. P. Landry, Y. S. Sun, X. W. Guo, and X. D. Zhu, "Protein reactions with surface-bound molecular targets detected by oblique-incidence reflectivity difference microscopes," Appl. Opt. 47, 3275-3288 (2008)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-47-18-3275

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited