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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 47, Iss. 19 — Jul. 1, 2008
  • pp: D96–D109

Phase, amplitude, and polarization microscopy with a sampling field sensor

Remy Tumbar  »View Author Affiliations


Applied Optics, Vol. 47, Issue 19, pp. D96-D109 (2008)
http://dx.doi.org/10.1364/AO.47.000D96


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Abstract

I describe an improved implementation of a previously reported interferometric device, the sampling field sensor (SFS) [ Appl. Opt. 47, B32–B43 (2008)]. It provides X, Y, and X Y shearing interferometric information simultaneously (space multiplexed) with amplitude and polarization information while using time-multiplexed phase shifting. Its simple common-path configuration makes it compact and vibration insensitive, as demonstrated by the λ / 125 phase estimation repeatability that was below the coherent noise floor (estimated at λ / 50 ). The SFS may be viewed as an efficient, robust and accurate full-field optical–digital interface, easy to integrate with traditional imaging systems. This is demonstrated by using the sensor as the focal plane array of a transmitted-light microscope in a straightforward setup using an illumination path polarization phase shifter. This work is focused on a qualitative demonstration and presents phase, amplitude, and polarization images of different types of human cheek cells and Caenorhabditiselegans larvae.

© 2008 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.0180) Medical optics and biotechnology : Microscopy
(180.3170) Microscopy : Interference microscopy
(350.5030) Other areas of optics : Phase
(090.1995) Holography : Digital holography

History
Original Manuscript: October 1, 2007
Revised Manuscript: December 21, 2007
Manuscript Accepted: January 14, 2008
Published: March 19, 2008

Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Remy Tumbar, "Phase, amplitude, and polarization microscopy with a sampling field sensor," Appl. Opt. 47, D96-D109 (2008)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-47-19-D96


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