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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 47, Iss. 2 — Jan. 10, 2008
  • pp: 110–115

Instrumentation for measuring fluorescence cross sections from airborne microsized particles

A. Manninen, M. Putkiranta, A. Rostedt, J. Saarela, T. Laurila, M. Marjamäki, J. Keskinen, and R. Hernberg  »View Author Affiliations

Applied Optics, Vol. 47, Issue 2, pp. 110-115 (2008)

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An experimental instrument for measuring a laser-induced fluorescence spectrum from a single aerosol particle is described. As a demonstration of instrument capabilities, the results of monodisperse 4.7 μ m sodium chloride particles doped with fluorescent riboflavin, produced with an inkjet aerosol generator, are presented. The fluorescence of the aerosol particles is excited in the wide range from 210 to 419   nm using a pulsed, tunable optical parametric oscillator laser. The maximum of the fluorescence emission of separately measured particles is detected at 560   nm . The dependence of the fluorescence on the excitation wavelength is studied and fluorescence cross sections are estimated. Agreement between the measured fluorescence data and the literature data for riboflavin is observed.

© 2008 Optical Society of America

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Coherence and Statistical Optics

Original Manuscript: May 24, 2007
Revised Manuscript: October 23, 2007
Manuscript Accepted: November 16, 2007
Published: January 7, 2008

Virtual Issues
Vol. 3, Iss. 2 Virtual Journal for Biomedical Optics

A. Manninen, M. Putkiranta, A. Rostedt, J. Saarela, T. Laurila, M. Marjamäki, J. Keskinen, and R. Hernberg, "Instrumentation for measuring fluorescence cross sections from airborne microsized particles," Appl. Opt. 47, 110-115 (2008)

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  1. J. Ho, M. Spence, and P. Hairston, "Measurement of biological aerosol with a fluorescent aerodynamic particle sizer (FLAPS): correlation of optical data with biological data," Aerobiologia 15, 281-291 (1999). [CrossRef]
  2. Y.-L. Pan, S. Holler, R. K. Chang, S. C. Hill, R. G. Pinnick, S. Niles, and J. R. Bottiger, "Single-shot fluorescence spectra of individual micrometer-sized bioaerosols illuminated by a 351- or a 266-nm ultraviolet laser," Opt. Lett. 24, 116-118 (1999). [CrossRef]
  3. G. Chen, P. Nachman, R. G. Pinnick, S. C. Hill, and R. K. Chang, "Conditional-firing aerosol-fluorescence spectrum analyzer for individual airborne particles with pulsed 266-nm laser excitation," Opt. Lett. 21, 1307-1309 (1996). [CrossRef] [PubMed]
  4. S. C. Hill, R. G. Pinnick, P. Nachman, G. Chen, R. K. Chang, M. W. Mayo, and G. L. Fernandez, "Aerosol-fluorescence spectrum analyzer: real-time measurement of emission spectra of airborne biological particles," Appl. Opt. 34, 7149-7155 (1995). [CrossRef] [PubMed]
  5. P. Jonsson, F. Kullander, P. Wästerby, M. Tiihonen, and M. Lindgren, "Detection of fluorescence spectra of individual bioaerosol particles," Proc. SPIE 5990, 59900M (2005). [CrossRef]
  6. P. Nachman, G. Chen, R. G. Pinnick, S. C. Hill, R. K. Chang, M. W. Mayo, and G. L. Fernandez, "Conditional-sampling spectrograph detection system for fluorescence measurements of individual airborne biological particles," Appl. Opt. 35, 1069-1076 (1996). [CrossRef] [PubMed]
  7. Y.-L. Pan, R. G. Pinnick, S. C. Hill, S. Niles, S. Holler, J. R. Bottiger, J.-P. Wolf, and R. K. Chang, "Dynamics of photon-induced degradation and fluorescence in riboflavin microparticles," Appl. Phys. B 72, 449-454 (2001).
  8. Y.-L. Pan, V. Boutou, and R. K. Chang, "Application of light-emitting diodes for aerosol fluorescence detection," Opt. Lett. 28, 1707-1709 (2003). [CrossRef] [PubMed]
  9. Y.-L. Pan, J. Hartings, R. G. Pinnick, S. C. Hill, J. Halverson, and R. K. Chang, "Single-particle fluorescence spectrometer for ambient aerosols," Aerosol Sci. Technol. 37, 627-638 (2003).
  10. R. G. Pinnick, S. C. Hill, S. Niles, D. M. Garvey, Y.-L. Pan, J. Bottiger, B. V. Bronk, B. T. Chen, C.-S. Orr, and G. Feather, "Real-time measurement of fluorescence spectra from single airborne biological particles," Field Anal. Chem. Technol. 3, 221-239 (1999). [CrossRef]
  11. M. A. Stowers, A. L. van Wuijckhuijse, J. C. M. Marijnissen, C. E. Kientz, and T. Ciach, "Fluorescence preselection of bioaerosol for single-particle mass spectrometry," Appl. Opt. 45, 8531-8536 (2006). [CrossRef] [PubMed]
  12. B. V. Bronk and L. Reinisch, "Variability of steady-state bacterial fluorescence with respect to growth conditions," Appl. Spectrosc. 47, 436-440 (1993). [CrossRef]
  13. G. W. Faris, R. A. Copeland, K. Mortelmans, and B. V. Bronk, "Resolved absolute fluorescence cross sections for bacillus spores," Appl. Opt. 36, 958-967 (1997). [CrossRef] [PubMed]
  14. V. Sivaprakasam, A. L. Huston, C. Scotto, and J. D. Eversole, "Multiple UV wavelength excitation and fluorescence of bioaerosols," Opt. Express 12, 4457-4466 (2004). [CrossRef] [PubMed]
  15. R. Weichert, W. Klemm, K. Legenhausen, and C. Pawellek, "Determination of fluorescence cross-sections of biological aerosols," Part. Part. Syst. Charact. 19, 216-222 (2002). [CrossRef]
  16. Y. S. Cheng, E. B. Barr, B. J. Fan, P. J. Hargis, J. P. J. Hargis, D. J. Rader, T. J. O'Hern, J. R. Torczynski, G. C. Tisone, B. L. Preppernan, S. A. Young, and R. J. Radloff, "Detection of bioaerosols using multiwavelength UV fluorescence spectroscopy," Aerosol Sci. Technol. 30, 186-201 (1999). [CrossRef]
  17. J. Kunnil, B. Swartz, and L. Reinisch, "Changes in the luminescence between dried and wet bacillus spores," Appl. Opt. 443, 5404-5409 (2004). [CrossRef]
  18. J. Bottiger, P. Deluca, E. Stuebing, and D. Vanreenen, "An ink jet aerosol generator," J. Aerosol Sci. Vol. , Suppl. I 29, S965-S966 (1998). [CrossRef]
  19. L. S. Dietrich and B. F. Harland, "Evidence indicating a chemical reaction between hydroxylamine and riboflavin," J. Biol. Chem. 217, 383-390 (1956).
  20. O. Svelto, Principle of Lasers, 4th ed. (Springer, 1998), Chap. 2.
  21. P. Jonsson, F. Kullander, M. Nordstrand, T. Tjärnhage, P. Wästerby, and M. Lindgren, "Development of fluorescence-based point detector for biological sensing," Proc. SPIE 5617, 60-74 (2004). [CrossRef]
  22. S. C. Hill, R. G. Pinnick, S. Niles, J. N. F. Fell, Y.-L. Pan, J. Bottiger, B. V. Bronk, S. Holler, and R. K. Chang, "Fluorescence from airborne microparticles: dependence on size, concentration of fluorophores, and illumination intensity," Appl. Opt. 40, 3005-3013 (2001). [CrossRef]

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