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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 47, Iss. 21 — Jul. 20, 2008
  • pp: 3789–3794

Effect of the polarization and incident angle of excitation light on the fluorescence enhancement observed with a multilayered substrate fabricated by Ag and Al 2 O 3

Takuo Akimoto, Mitsuru Yasuda, and Isao Karube  »View Author Affiliations


Applied Optics, Vol. 47, Issue 21, pp. 3789-3794 (2008)
http://dx.doi.org/10.1364/AO.47.003789


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Abstract

The fluorescence from a fluorophore on a multilayered substrate fabricated by a metal and a dielectric is known to be enhanced by more than 100-fold. In the course of this study, we prepared a multilayered substrate with Ag as the metal and Al 2 O 3 as the dielectric and then investigated the effects of the polarization of the excitation light on the enhancement of the multilayered substrate. It was found that the enhancement was attributed to an electric field oscillating parallel to the substrate. Maximum 200-fold enhancement could be achieved with 80 nm thick Al 2 O 3 when an unpolarized excitation light was used with an incident angle of 20 ° .

© 2008 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(260.2510) Physical optics : Fluorescence
(310.6845) Thin films : Thin film devices and applications

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 5, 2008
Revised Manuscript: May 18, 2008
Manuscript Accepted: June 13, 2008
Published: July 14, 2008

Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Takuo Akimoto, Mitsuru Yasuda, and Isao Karube, "Effect of the polarization and incident angle of excitation light on the fluorescence enhancement observed with a multilayered substrate fabricated by Ag and Al2O3," Appl. Opt. 47, 3789-3794 (2008)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-47-21-3789


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References

  1. J. R. Lakowicz, “Radiative decay engineering: biophysical and biomededical applications,” Anal. Biochem. 298, 1-24 (2001). [CrossRef] [PubMed]
  2. J. R. Lakowicz, Y. Shen, S. D'Auria, J. Malicka, J. Fang, Z. Gryczynski, and I. Gryczynski, “Radiative decay engineering: 2. Effect of silver island films on fluorescence intensity, lifetime, and resonance energy transfer,” Anal. Biochem. 301, 261-277 (2002). [CrossRef] [PubMed]
  3. J. R. Lakowicz, C. D. Geddes, I. Gryczynski, J. Malicka, Z. Gryczynski, K. Aslan, J. Lukomska, E. Matveeva, J. Zhang, R. Badugu, and J. Huang, “Advances in surface-enhanced fluorescence,” J. Fluoresc. 14, 425-441 (2004). [CrossRef] [PubMed]
  4. P. J. Tarcha, J. Desaja-Gonzalez, S. Rodriguez-Llorente, and R. Aroca, “Surface-enhanced fluorescence on SiO2-coated silver island films,” Appl. Spectrosc. 53, 43-48 (1999). [CrossRef]
  5. R. M. Bakker, H.-K. Yuan, Z. Liu, V. P. Drachev, A. V. Kildishev, V. M. Shalaev, R. H. Pedersen, S. Gresillon, and A. Boltasseva, “Enhanced localized fluorescence in plasmonic nanoantennae,” Appl. Phys. Lett. 92, 043101 (2008). [CrossRef]
  6. J. Malicka, I. Gryczynski, Z. Gryczynski, and J. R. Lakowicz, “Effect of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides,” Anal. Biochem. 315, 57-66 (2003). [CrossRef] [PubMed]
  7. J. R. Lakowicz, J. Kuśba, Y. Shen, J. Malicka, S. D'Auria, Z. Gryczynski, and I. Gryczynski, “Effects of metallic silver particles on resonance energy transfer between fluorophores bound to DNA,” J. Fluoresc. 13, 69-77 (2003). [CrossRef]
  8. Y. Fu and J. R. Lakowicz, “Enhanced fluorescence of Cy5-labeled oligonucleotides near silver island films: a distance effect study using single molecular spectroscopy,” J. Phys. Chem. B 110, 22557-22562 (2006). [CrossRef] [PubMed]
  9. Y. Fu and J. R. Lakowicz, “Enhanced fluorescence of Cy5-labeled DNA tethered to silver island films: fluorescence image and time-resolved studies using single-molecule spectroscopy,” Anal. Chem. 78, 6238-6245 (2006). [CrossRef] [PubMed]
  10. C. R. Sabanayagam and J. R. Lakowicz, “Increasing the sensitivity of DNA microarrays by metal-enhanced fluorescence using surface-bound silver nanoparticles,” Nucleic Acids Res. 35, e13 (2007). [CrossRef]
  11. J. Zhnag and J. R. Lakowicz, “A model for DNA detection by metal-enhanced fluorescence from immobilized silver nanoparticles on solid substrate,” J. Phys. Chem. B 110, 2387-2392 (2006). [CrossRef]
  12. E. G. Matveeva, Z. Gryczynski, and J. R. Lakowicz, “Myoglobin immunoassay based on metal particle-enhanced fluorescence,” J. Immunol. Methods 302, 26-35 (2005). [CrossRef] [PubMed]
  13. J. Zhang, E. Matveeva, I. Gryczynski, Z. Leonenko, and J. R. Lakowicz, “Metal-enhanced fluoroimmunoassay on a silver film by vapor deposition,” J. Phys. Chem. B 109, 7969-7957(2005). [CrossRef]
  14. E. G. Matveeva, I. Gryczynski, A. Barnett, Z. Leonenko, J. R. Lakowicz, and Z. Gryczynski, “Metal particle-enhanced fluorescent immunoassay on metal mirrors,” Anal. Biochem. 363, 239-245 (2007). [CrossRef] [PubMed]
  15. K. H. Drexhage, “Interaction of light with monomolecular dye layers,” in Progress in Optics, E. Wolf, ed. (North-Holland, 1974), Vol. 12, pp. 163-232. [CrossRef]
  16. W. R. Holland and D. G. Hall, “Waveguide mode enhancement of molecular fluorescence,” Opt. Lett. 10, 414-416 (1985). [CrossRef] [PubMed]
  17. K. G. Sullivan, O. King, C. Sigg, and D. G. Hall, “Directional, enhanced fluorescence from molecular near aperiodic surface,” Appl. Opt. 33, 2447-2454 (1994). [CrossRef] [PubMed]
  18. K. G. Sullivan and D. G. Hall, “Enhancement and inhibition of electromagnetic radiation in plane-layered media. I. Plane-wave-spectrum approach to modeling classical effects,” J. Opt. Soc. Am. B 14, 1149-1159 (1997). [CrossRef]
  19. K. G. Sullivan and D. G. Hall, “Enhancement and inhibition of electromagnetic radiation in plane-layered media. II. Enhanced fluorescence in optical waveguide sensors,” J. Opt. Soc. Am. B 14, 1160-1166 (1997). [CrossRef]
  20. L. Novotny and B. Hecht, Principles of Nano-Optics (Cambridge University Press, 2006), Chap. 10.

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