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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 47, Iss. 22 — Aug. 1, 2008
  • pp: 4164–4176

Simultaneous measurement of changes in light absorption due to the reduction of cytochrome c oxidase and light scattering in rat brains during loss of tissue viability

Satoko Kawauchi, Shunichi Sato, Hidetoshi Ooigawa, Hiroshi Nawashiro, Miya Ishihara, and Makoto Kikuchi  »View Author Affiliations


Applied Optics, Vol. 47, Issue 22, pp. 4164-4176 (2008)
http://dx.doi.org/10.1364/AO.47.004164


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Abstract

We performed the simultaneous measurement of intrinsic optical signals (IOSs) related to metabolic activity and cellular and subcellular morphological characteristics, i.e., light scattering for a rat global ischemic brain model made by rapidly removing blood by saline infusion. The signals were measured on the basis of multiwavelength diffuse reflectances in which 605 and 830 nm were used to detect the IOSs that are thought to be dominantly affected by redox changes of heme a a 3 and CuA in cytochrome c oxidase (CcO), respectively. For measuring the scattering signal, the wavelength that was found to be most insensitive to the absorption changes, e.g., 620 nm , was used. The measurements suggested that an increase in the absorption due to reduction of heme a a 3 occurred soon after blood clearance, and this was followed by a large triphasic change in light scattering, during which time a decrease in the absorption due to reduction of CuA occurred. Through the triphasic scattering change, scattering signals increased by 5.2 ± 1.5 % ( n = 5 ), and the increase in light scattering showed significant correlation with both the reflectance intensity changes at 605 and 830 nm . This suggests that morphological changes in cells correlate with reductions of heme a a 3 and CuA. Histological analysis of tissue after the triphasic scattering change showed no alteration in either the nuclei or the cytoskeleton, but electron microscopic observation revealed deformed, enlarged mitochondria and expanded dendrites. These findings suggest that the simultaneous measurement of absorption signals related to the redox changes in the CcO and the scattering signal is useful for monitoring tissue viability in the brain.

© 2008 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 1, 2007
Revised Manuscript: May 29, 2008
Manuscript Accepted: July 7, 2008
Published: July 30, 2008

Virtual Issues
Vol. 3, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Satoko Kawauchi, Shunichi Sato, Hidetoshi Ooigawa, Hiroshi Nawashiro, Miya Ishihara, and Makoto Kikuchi, "Simultaneous measurement of changes in light absorption due to the reduction of cytochrome c oxidase and light scattering in rat brains during loss of tissue viability," Appl. Opt. 47, 4164-4176 (2008)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-47-22-4164

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