Scanning flow cytometer modified to distinguish phytoplankton cells from their effective size, effective refractive index, depolarization, and fluorescence
Applied Optics, Vol. 47, Issue 24, pp. 4405-4412 (2008)
http://dx.doi.org/10.1364/AO.47.004405
Enhanced HTML 
Acrobat PDF (7756 KB)
Abstract
A laser flow cytometer based on scanning flow cytometry has been assembled. The unpolarized and linearly polarized light-scattering profiles, as well as the side emitted light in different spectral bands, were measured, allowing the simultaneous and real-time determination of the effective size and the effective refractive index of each spherelike particle. Additionally, each particle could be identified from depolarization and fluorescence measured simultaneously. The tests with aqueous samples of polystyrene spheres, fluorescent or nonfluorescent, and phytoplankton cells demonstrate that the system is able to retrieve size and refractive index with an accuracy of 1% and that the depolarization and fluorescence measurements allow the classification of particles otherwise indistinguishable.
© 2008 Optical Society of America
OCIS Codes
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(170.1530) Medical optics and biotechnology : Cell analysis
(300.2530) Spectroscopy : Fluorescence, laser-induced
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: May 7, 2008
Manuscript Accepted: July 13, 2008
Published: August 19, 2008
Virtual Issues
Vol. 3, Iss. 10 Virtual Journal for Biomedical Optics
Citation
Luca Fiorani, Valeri P. Maltsev, Vyacheslav M. Nekrasov, Antonio Palucci, Konstantin A. Semyanov, and Valeria Spizzichino, "Scanning flow cytometer modified to distinguish phytoplankton cells from their effective size, effective refractive index, depolarization, and fluorescence," Appl. Opt. 47, 4405-4412 (2008)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-47-24-4405
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Simultaneous Light Scattering and Intrinsic Fluorescence Measurement for the Classification of Airborne Particles (AO)
- Rayleigh-Calibrated Fluorescence Quantum Yield Measurements of Acetone and 3-Pentanone (AO)
- Experimental and theoretical study of light scattering by individual mature red blood cells by use of scanning flow cytometry and a discrete dipole approximation (AO)
- Unified Mie and fractal scattering by biological cells and subcellular structures (OL)
- Quantification of the evaporative cooling in an ethanol spray created by a gasoline direct-injection system measured by multiline NO-LIF gas-temperature imaging (AO)
Related Conference Papers
- Development of Laser Induced Fluorescence Sensor for the Detection of Human Breast Cancer Cell lines Conjugated with Gold Nanoparticles
- Single-fiber for minimally-invasive identification and collection of biological species
- Single-fiber for minimally-invasive identification and collection of biological species
- Single-fiber for minimally-invasive identification and collection of biological species
- Micro-fluidic based detection platform for rapid pathogen characterization in water
« Previous Article | Next Article »
OSA is a member of 