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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 47, Iss. 31 — Nov. 1, 2008
  • pp: 5944–5952

In vivo fluorescent optical imaging of cytotoxic T lymphocyte migration using IRDye800CW near-infrared dye

Aaron E. Foster, Sunkuk Kwon, Shi Ke, An Lu, Karen Eldin, Eva Sevick-Muraca, and Cliona M. Rooney  »View Author Affiliations


Applied Optics, Vol. 47, Issue 31, pp. 5944-5952 (2008)
http://dx.doi.org/10.1364/AO.47.005944


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Abstract

We describe a method to measure in vivo migration of human T cells by using the near-infrared (NIR) dye IRDye800CW. Labeling of Epstein–Barr virus-specific T cells with IRDye800CW did not affect viability, proliferation, or T cell function. Following tail vein injection into mice bearing subcutaneous tumors, the NIR signal could be measured in vivo at the tumor site. Analysis of tumors revealed T cell infiltration and an increased NIR signal, confirming T cell migration. To test specific migration with IRDye800CW, tumors were modified to express CCL5 to measure site-specific migration. The NIR signal was increased at CCL5-secreting tumors compared with control tumors. Together, these data suggest that IRDye800CW may be used to study the trafficking of T cells in a small animal model and may have potential as a short-term reporter molecule for human immunotherapy studies.

© 2008 Optical Society of America

OCIS Codes
(110.3080) Imaging systems : Infrared imaging
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1420) Medical optics and biotechnology : Biology
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: June 9, 2008
Revised Manuscript: September 9, 2008
Manuscript Accepted: September 10, 2008
Published: October 31, 2008

Virtual Issues
Vol. 4, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Aaron E. Foster, Sunkuk Kwon, Shi Ke, An Lu, Karen Eldin, Eva Sevick-Muraca, and Cliona M. Rooney, "In vivo fluorescent optical imaging of cytotoxic T lymphocyte migration using IRDye800CW near-infrared dye," Appl. Opt. 47, 5944-5952 (2008)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-47-31-5944


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