Laser-induced breakdown spectroscopy (LIBS) was used to discern between two biological agent surrogates (Bacillus atrophaeus and ovalbumin) and potential interferent compounds (mold spores, humic acid, house dust, and Arizona road dust). Multiple linear regression and neural network analysis models were constructed by using B. atrophaeus and ovalbumin spectra, and limits of detection were calculated. Classification of the agent surrogates’ LIBS spectra was attempted by using a neural network model. False negative rates of 0% were observed for B. atrophaeus (100 colony forming units) spore spectra with the neural network model used for classification.
© 2008 Optical Society of America
Original Manuscript: March 27, 2008
Revised Manuscript: June 18, 2008
Manuscript Accepted: June 26, 2008
Published: September 4, 2008
Vol. 4, Iss. 1 Virtual Journal for Biomedical Optics
Emily Gibb Snyder, Chase A. Munson, Jennifer L. Gottfried, Frank C. De Lucia, Jr., Brian Gullett, and Andrzej Miziolek, "Laser-induced breakdown spectroscopy for the classification of unknown powders," Appl. Opt. 47, G80-G87 (2008)