There are two goals in this simulation study: (1) to show that the time variation of the bioluminescence source can cause artifacts in the tomographic images such that quantification and localization becomes impossible; and (2) to show that the a priori knowledge of the light kinetics can be used to eliminate these artifacts. These goals are motivated by the fact that the half-life of luciferase has been reported as 30 min to 2 h in vivo. We perform two-dimensional simulations. We consider a 40 mm diameter circular region with an inclusion of 6 mm diameter located 10 mm away from the center. The measurement data is simulated using a finite-element-based forward solver. We model the noncontact measurements such that four-wavelength data is collected from four 90° apart views. The results show that the ratio of the total imaging time to the half-life of the exponentially decaying bioluminescent source is the deciding factor in the reconstruction of the source. It is also demonstrated that a priori knowledge of the source kinetics is required to perform tomographic bioluminescence imaging of short half-life bioluminescent sources and the use of spatial a priori information alone is not adequate.
© 2008 Optical Society of America
Medical Optics and Biotechnology
Original Manuscript: August 9, 2007
Manuscript Accepted: November 10, 2007
Published: February 19, 2008
Vol. 3, Iss. 3 Virtual Journal for Biomedical Optics
Mehmet Burcin Unlu and Gultekin Gulsen, "Effects of the time dependence of a bioluminescent source on the tomographic reconstruction," Appl. Opt. 47, 799-806 (2008)