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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 47, Iss. 6 — Feb. 20, 2008
  • pp: 825–834

Autofluorescence and diffuse reflectance spectroscopy of oral epithelial tissue using a depth-sensitive fiber-optic probe

Richard A. Schwarz, Wen Gao, Dania Daye, Michelle D. Williams, Rebecca Richards-Kortum, and Ann M. Gillenwater  »View Author Affiliations

Applied Optics, Vol. 47, Issue 6, pp. 825-834 (2008)

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Optical spectroscopy can provide useful diagnostic information about the morphological and biochemical changes related to the progression of precancer in epithelial tissue. As precancerous lesions develop, the optical properties of both the superficial epithelium and underlying stroma are altered; measuring spectral data as a function of depth has the potential to improve diagnostic performance. We describe a clinical spectroscopy system with a depth-sensitive, ball lens coupled fiber-optic probe for noninvasive in vivo measurement of oral autofluorescence and diffuse reflectance spectra. We report results of spectroscopic measurements from oral sites in normal volunteers and in patients with neoplastic lesions of the oral mucosa; results indicate that the addition of depth selectivity can enhance the detection of optical changes associated with precancer.

© 2008 Optical Society of America

OCIS Codes
(060.2310) Fiber optics and optical communications : Fiber optics
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: August 20, 2007
Revised Manuscript: December 13, 2007
Manuscript Accepted: December 27, 2007
Published: February 20, 2008

Virtual Issues
Vol. 3, Iss. 3 Virtual Journal for Biomedical Optics

Richard A. Schwarz, Wen Gao, Dania Daye, Michelle D. Williams, Rebecca Richards-Kortum, and Ann M. Gillenwater, "Autofluorescence and diffuse reflectance spectroscopy of oral epithelial tissue using a depth-sensitive fiber-optic probe," Appl. Opt. 47, 825-834 (2008)

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