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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 48, Iss. 10 — Apr. 1, 2009
  • pp: D151–D162

Optical calibration protocol for an x-ray and optical multimodality tomography system dedicated to small-animal examination

Anabela Da Silva, Mehdi Leabad, Clémence Driol, Thomas Bordy, Mathieu Debourdeau, Jean-Marc Dinten, Philippe Peltié, and Philippe Rizo  »View Author Affiliations

Applied Optics, Vol. 48, Issue 10, pp. D151-D162 (2009)

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A small-animal multimodality tomography system dedicated to the coregistration of fluorescence optical signal and x-ray measurements has been developed in our laboratory. The purpose of such a system is to offer the possibility of getting in vivo anatomical and functional information simultaneously. Moreover, anatomical measurements can be used as a regularization factor to achieve more accurate reconstructions of the biodistribution of fluorochromes and to speed up treatment. A dedicated acquisition protocol has been established, and the methodology of the reconstruction of the three-dimensional distribution of the biomarkers under cylindrical geometry consistent with classic computed tomography has been implemented. A phantom study was conducted to evaluate and to fix the parameters for the coregistration. These test experiments were reproduced by considering anesthetized mice that had thin glass tubes containing fluorochromes inserted into their esophagus. The instrument is also used for an in vivo biological study conducted on mice with lung tumors, tagged with near-infrared optical probes (targeting probes such as Transferin-AlexaFluor750).

© 2009 Optical Society of America

OCIS Codes
(110.4190) Imaging systems : Multiple imaging
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.7440) Medical optics and biotechnology : X-ray imaging
(110.6955) Imaging systems : Tomographic imaging

Original Manuscript: September 3, 2008
Revised Manuscript: February 4, 2009
Manuscript Accepted: February 6, 2009
Published: February 27, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Anabela Da Silva, Mehdi Leabad, Clémence Driol, Thomas Bordy, Mathieu Debourdeau, Jean-Marc Dinten, Philippe Peltié, and Philippe Rizo, "Optical calibration protocol for an x-ray and optical multimodality tomography system dedicated to small-animal examination," Appl. Opt. 48, D151-D162 (2009)

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