Simultaneous imaging of cerebral partial pressure of oxygen and blood flow during functional activation and cortical spreading depression
Applied Optics, Vol. 48, Issue 10, pp. D169-D177 (2009)
http://dx.doi.org/10.1364/AO.48.00D169
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Abstract
We developed a novel imaging technique that provides real-time two-dimensional maps of the absolute partial pressure of oxygen and relative cerebral blood flow in rats by combining phosphorescence lifetime imaging with laser speckle contrast imaging. Direct measurement of blood oxygenation based on phosphorescence lifetime is not significantly affected by changes in the optical parameters of the tissue during the experiment. The potential of the system as a novel tool for quantitative analysis of the dynamic delivery of oxygen to support brain metabolism was demonstrated in rats by imaging cortical responses to forepaw stimulation and the propagation of cortical spreading depression waves. This new instrument will enable further study of neurovascular coupling in normal and diseased brain.
© 2009 Optical Society of America
OCIS Codes
(110.6150) Imaging systems : Speckle imaging
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
History
Original Manuscript: September 3, 2008
Revised Manuscript: January 9, 2009
Manuscript Accepted: January 20, 2009
Published: February 27, 2009
Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics
Citation
Sava Sakadžić, Shuai Yuan, Ergin Dilekoz, Svetlana Ruvinskaya, Sergei A. Vinogradov, Cenk Ayata, and David A. Boas, "Simultaneous imaging of cerebral partial pressure of oxygen and blood flow during functional activation and cortical spreading depression," Appl. Opt. 48, D169-D177 (2009)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-48-10-D169
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