OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 48, Iss. 10 — Apr. 1, 2009
  • pp: D52–D61

Multichannel spectroreflectometry: a noninvasive method for assessment of on-line hemoglobin derivatives

Vasile Diaconu  »View Author Affiliations


Applied Optics, Vol. 48, Issue 10, pp. D52-D61 (2009)
http://dx.doi.org/10.1364/AO.48.000D52


View Full Text Article

Enhanced HTML    Acrobat PDF (887 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

The goal of the current study was to introduce a mathematical method to derive hemoglobin, oxyhemoglobin and carboxyl-hemoglobin absorption factors from full spectrum reflectometry measurements of retinal microcapillaries. The mathematical equation that describes the spectral reflectometry function was expressed as a linear combination of several terms of S i ( λ ) representing the spectral signature functions of hemoglobin, oxyhemoglobin, carboxyl-hemoglobin, ocular media, melanin, and a scattering factor. Contrary to the classical model, where the reflectometry function was expressed as an absorbance Ab ( λ ) = log ( incident light ( λ ) / reflected light ( λ ) ) , in this model and system, it is proposed to express the reflectometry function from the eye structures as an absorption factor A ( λ ) % = incident light ( λ ) / reflected light ( λ ) . To increase confidence in the estimation of hemoglobin derivatives, the mathematical model was applied to only a part of the spectral function of reflectometry, while the results of the model were used to explain the other part of the reflectometry function. The results demonstrate that for the visible spectral field, the model that explains the absorption of the light by the blood contained in the microcapillaries of biological structures is not compatible with the Beer–Lambert law.

© 2009 Optical Society of America

OCIS Codes
(170.1460) Medical optics and biotechnology : Blood gas monitoring
(170.3890) Medical optics and biotechnology : Medical optics instrumentation

History
Original Manuscript: June 19, 2008
Revised Manuscript: November 14, 2008
Manuscript Accepted: December 5, 2008
Published: January 12, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Vasile Diaconu, "Multichannel spectroreflectometry: a noninvasive method for assessment of on-line hemoglobin derivatives," Appl. Opt. 48, D52-D61 (2009)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-48-10-D52


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. J. B. Hickham, R. Frayser, and J. C. Ross, “A study of retinal venous blood oxygen saturation in human subjects by photographic means,” Circulation 27, 375-85 (1963).
  2. R. A. Laing, L. A. Danisch, and L. R. Young, “The choroidal eye oximeter: an instrument for measuring oxygen saturation of choroidal blood in vivo,” IEEE Trans. Biomed. Eng. BME-22, 183-95 (1975). [CrossRef]
  3. F. C. Delori and K. P. Pflibsen, “Spectral reflectance of the human ocular fundus,” Appl. Opt. 28, 1061-1076 (1989). [CrossRef] [PubMed]
  4. D. Schweitzer, L. Leistritz, M. Hammer, and M. Scibor, “Calibration-free measurement of the oxygen saturation in retinal vessels of men,” Proc. SPIE 2393, 210-218 (1995) [CrossRef]
  5. D. Schweitzer, E. Thamm, M. Hammer, and J. Kraft, “A new method for the measurement of oxygen saturation at the human ocular fundus,” Int. Ophthalmol. 23347-53 (2001). [CrossRef]
  6. J. Foubert and V. Diaconu, “On-line and real-time spectroreflectometry measurement of oxygenation in a patient's eye,” U.S. patent 5,919,132 (6 July 1999).
  7. J. Faubert, V. Diaconu, M. Ptito, and A. Ptito, “Residual vision in the blind field of hemidecorticated humans predicted by a diffusion scatter model and selective spectral absorption of the human eye,” Vision Res. 39, 149-157 (1999). [CrossRef] [PubMed]
  8. D. Van Norren and L. F. Tiemeijer, “Spectral reflectance of the human eye,” Vision Res. 26, 313-320 (1986). [CrossRef] [PubMed]
  9. M. Hammer, E. Thamm, and D. Schweitzer, “A simple algorithm for in vivo ocular fundus oximetry compensating for non-haemoglobin absorption and scattering,” Phys. Med. Biol. 47, N233-N238 (2002). [CrossRef] [PubMed]
  10. R. N. Pittman and B. R. Duling, “Measurement of percent oxyhemoglobin in the microvasculature,” J. Appl. Physiol. 38, 321-327 (1975). [PubMed]
  11. H. J. A. Dartnell, “The interpretation of spectral sensitivity curves,” Br. Med. Bull. 9, 24-36 (1953).
  12. O. W. Van Assendelft and W. G. Zijlstra, “Extinction coefficients for use in equations for the spectrophotometric analysis of haemoglobin mixtures,” Anal. Biochem. 69, 43-48 (1975) [CrossRef] [PubMed]
  13. J. Xu, J. Pokorny, and V. Smith, “Optical density of the human lens,” J. Opt. Soc. Am. A 14, 953-960 (1997). [CrossRef]
  14. N. P. A. Zagers and D. Van Norren, “Absorption of the eye lens and macular pigment derived from the reflectance of cone photoreceptors,” J. Opt. Soc. Am. A 21, 2257-2268(2004). [CrossRef]
  15. F. C. Delori and S. A. Burns, “Fundus reflectance and the measurement of crystalline lens density,” J. Opt. Soc. Am. A 13, 215-226 (1996). [CrossRef]
  16. J. J. Weiter, F. C. Delori, G. L. Wing, and K. A. Fitch, “Retinal pigment epithelial lipofuscin and melanin and choroidal melanin in human eyes,” Invest. Ophthalmol. Visual Sci. 27, 145-152 (1986).
  17. V. P. Gabel, R. Birngruber, and F. Hillenkamp, “Visible and near infrared light absorption in pigment epithelium and choroid,” Proceedings of XXIII Consilium Ophthalmologicum, Kyoto, K. Shimizu, J. A. Osterhuis eds. (Amsterdam Oxford: Excerpta Medica, 1978), pp. 658-662.
  18. I. A. Menon, S. Persad, H. F. Haberman, C. J. Kurian, and P. K. Basu, “A qualitative study of the melanins from blue end brown human eyes,” Exp. Eye Res. 34, 531-537(1982). [CrossRef] [PubMed]
  19. W. J. Geeraets, R. C. Williams, G. Chan, and W. T. Ham, “The relative absorption of thermal energy in retina and choroid,” Invest. Ophthalmol. Visual Sci. 1, 340-347 (1962).
  20. S. Suner, “Non-invasive pulse CO-oximetry screening in the emergency department identifies occult carbon monoxide toxicity,” J. Emerg. Med. 34, 441-450. [PubMed]
  21. D. Schweitzer, “In vivo measurement of the oxygen saturation of retinal vessels in healthy volunteers,” IEEE Trans. Biomed. Eng. 46, 1454-1465 (1999). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited