OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 48, Iss. 10 — Apr. 1, 2009
  • pp: D88–D95

Ex vivo and in vivo second-harmonic-generation imaging of dermal collagen fiber in skin: comparison of imaging characteristics between mode-locked Cr:forsterite and Ti:sapphire lasers

Takeshi Yasui, Yu Takahashi, Masahiro Ito, Shuichiro Fukushima, and Tsutomu Araki  »View Author Affiliations


Applied Optics, Vol. 48, Issue 10, pp. D88-D95 (2009)
http://dx.doi.org/10.1364/AO.48.000D88


View Full Text Article

Enhanced HTML    Acrobat PDF (1143 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Second-harmonic-generation (SHG) microscopy is an interesting new tool for observing dermal collagen fiber in skin. However, conventional SHG microscopy using a mode-locked Ti:sapphire laser suffers from low penetration depth and a slow image acquisition rate caused by scattering and absorption in tissue, making it difficult to use for in vivo applications on human skin. We develop an SHG microscope equipped with a mode-locked Cr:forsterite laser with a long wavelength and compare its imaging characteristics with that of a Ti:sapphire-laser-based SHG microscope for the measurement of dermal collagen fiber in animal and human skins. The results indicate the suitability of the Cr:forsterite laser-based SHG microscope for in vivo imaging of human skin.

© 2009 Optical Society of America

OCIS Codes
(170.1870) Medical optics and biotechnology : Dermatology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6960) Medical optics and biotechnology : Tomography
(190.4160) Nonlinear optics : Multiharmonic generation
(180.4315) Microscopy : Nonlinear microscopy

History
Original Manuscript: September 2, 2008
Revised Manuscript: December 22, 2008
Manuscript Accepted: December 26, 2008
Published: January 26, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Takeshi Yasui, Yu Takahashi, Masahiro Ito, Shuichiro Fukushima, and Tsutomu Araki, "Ex vivo and in vivo second-harmonic-generation imaging of dermal collagen fiber in skin: comparison of imaging characteristics between mode-locked Cr:forsterite and Ti:sapphire lasers," Appl. Opt. 48, D88-D95 (2009)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-48-10-D88

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Supplementary Material


» Media 1: MOV (3788 KB)     
» Media 2: MOV (2540 KB)     
» Media 3: MOV (2991 KB)     
» Media 4: MOV (3245 KB)     

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited