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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 48, Iss. 11 — Apr. 10, 2009
  • pp: 2067–2077

High-resolution mosaic imaging with multifocal, multiphoton photon-counting microscopy

Eric Chandler, Erich Hoover, Jeff Field, Kraig Sheetz, Wafa Amir, Ramon Carriles, Shi-you Ding, and Jeff Squier  »View Author Affiliations


Applied Optics, Vol. 48, Issue 11, pp. 2067-2077 (2009)
http://dx.doi.org/10.1364/AO.48.002067


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Abstract

High-resolution mosaic imaging is performed for the first time to our knowledge with a multifocal, multiphoton, photon-counting imaging system. We present a novel design consisting of a home-built femtosecond Yb-doped KGdWO 4 laser with an optical multiplexer, which is coupled with a commercial Olympus IX-71 microscope frame. Photon counting is performed using single-element detectors and an inexpensive electronic demultiplexer and counters.

© 2009 Optical Society of America

OCIS Codes
(030.5260) Coherence and statistical optics : Photon counting
(180.5810) Microscopy : Scanning microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: October 21, 2008
Revised Manuscript: February 11, 2009
Manuscript Accepted: February 13, 2009
Published: April 2, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Eric Chandler, Erich Hoover, Jeff Field, Kraig Sheetz, Wafa Amir, Ramon Carriles, Shi-you Ding, and Jeff Squier, "High-resolution mosaic imaging with multifocal, multiphoton photon-counting microscopy," Appl. Opt. 48, 2067-2077 (2009)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-48-11-2067


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