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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 48, Iss. 11 — Apr. 10, 2009
  • pp: 2067–2077

High-resolution mosaic imaging with multifocal, multiphoton photon-counting microscopy

Eric Chandler, Erich Hoover, Jeff Field, Kraig Sheetz, Wafa Amir, Ramon Carriles, Shi-you Ding, and Jeff Squier  »View Author Affiliations

Applied Optics, Vol. 48, Issue 11, pp. 2067-2077 (2009)

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High-resolution mosaic imaging is performed for the first time to our knowledge with a multifocal, multiphoton, photon-counting imaging system. We present a novel design consisting of a home-built femtosecond Yb-doped KGdWO 4 laser with an optical multiplexer, which is coupled with a commercial Olympus IX-71 microscope frame. Photon counting is performed using single-element detectors and an inexpensive electronic demultiplexer and counters.

© 2009 Optical Society of America

OCIS Codes
(030.5260) Coherence and statistical optics : Photon counting
(180.5810) Microscopy : Scanning microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: October 21, 2008
Revised Manuscript: February 11, 2009
Manuscript Accepted: February 13, 2009
Published: April 2, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Eric Chandler, Erich Hoover, Jeff Field, Kraig Sheetz, Wafa Amir, Ramon Carriles, Shi-you Ding, and Jeff Squier, "High-resolution mosaic imaging with multifocal, multiphoton photon-counting microscopy," Appl. Opt. 48, 2067-2077 (2009)

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  1. J. Bewersdorf, R. Pick, and S. Hell, “Multifocal multiphoton microscopy,” Opt. Lett. 23, 655-657 (1998). [CrossRef]
  2. M. Straub and S. Hell, “Multifocal multiphoton microscopy: a fast and efficient tool for 3-D fluorescence imaging,” Bioimaging 6, 177-185 (1998).
  3. A. Egner and S. Hell, “Time multiplexing and parallelization in multifocal multiphoton microscopy,” J. Opt. Soc. Am. A 17, 1192-1201 (2000). [CrossRef]
  4. A. Buist, M. Müller, J. Squier, and G. Brakenhoff, “Real time two-photon absorption microscopy using multi-point excitation,” J. Microsc. (Oxford) 192, 217-226 (1998). [CrossRef]
  5. K. Bahlmann, P. So, M. Kirber, R. Reich, B. Kosicki, W. McGonagle, and K. Bellve, “Multifocal multiphoton microscopy (MMM) at a frame rate beyond 600 Hz,” Opt. Express 15, 10991 (2007). [CrossRef] [PubMed]
  6. V. Andresen, A. Egner, and S. Hell, “Time-multiplexed multifocal multiphoton microscope,” Opt. Lett. 26, 75-77 (2001). [CrossRef]
  7. K. Kim, C. Buehler, K. Bahlmann, T. Ragan, W. Lee, E. Nedivi, E. Heffer, S. Fantini, and P. So, “Multifocal multiphoton microscopy based on multianode photomultiplier tubes,” Opt. Express 15, 11658-11678 (2007). [CrossRef] [PubMed]
  8. D. Fittinghoff and J. Squier, “Time-decorrelated multifocal array for multiphoton microscopy and micromachining,” Opt. Lett. 25, 1213-1215 (2000). [CrossRef]
  9. R. Niesner, V. Andresen, J. Neumann, H. Spiecker, and M. Gunzer, “The power of single and multibeam two-photon microscopy for high-resolution and high-speed deep tissue and intravital imaging,” Biophys. J. 93, 2519-2529 (2007). [CrossRef] [PubMed]
  10. J. Martini, K. Schmied, R. Palmisano, K. Toensing, D. Anselmetti, and T. Merkle, “Multifocal two-photon laser scanning microscopy combined with photo-activatable GFP for in vivo monitoring of intracellular protein dynamics in real time,” J. Struct. Biol. 158, 401-409 (2007). [CrossRef] [PubMed]
  11. S. Kumar, C. Dunsby, P. De Beule, D. Owen, U. Anand, P. Lanigan, R. Benninger, D. Davis, M. Neil, P. Anand, C. Benham, A. Naylor, and P. French, “Multifocal multiphoton excitation and time correlated single photon counting detecting for 3-D fluorescence lifetime imaging,” Opt. Express 15, 12548-12561 (2007). [CrossRef] [PubMed]
  12. D. Fittinghoff, C. Schaffer, E. Mazur, and J. Squier, “Time-decorrelated multifocal micromachining and trapping,” IEEE J. Sel. Top. Quantum Electron. 7, 559-566 (2001). [CrossRef]
  13. J. Jureller, H. Kim, and N. Scherer, “Stochastic scanning multiphoton multifocal microscopy,” Opt. Express 14, 3406-3414(2006). [CrossRef] [PubMed]
  14. L. Sacconi, E. Froner, R. Antolini, M. Taghizadeh, A. Choudhury, and F. Pavone, “Multiphoton multifocal microscopy exploiting a diffractive optical element,” Opt. Lett. 28, 1918-1920 (2003). [CrossRef] [PubMed]
  15. W. Amir, R. Carriles, E. Hoover, T. Planchon, C. Durfee, and J. Squier, “Simultaneous imaging of multiple focal planes using a two-photon scanning microscope,” Opt. Lett. 32, 1731-1733 (2007). [CrossRef] [PubMed]
  16. R. Carriles, K. Sheetz, E. Hoover, J. Squier, and V. Barzda, “Simultaneous multifocal, multiphoton, photon counting microscopy,” Opt. Express 16, 10364-10371 (2008). [CrossRef] [PubMed]
  17. K. Sheetz, E. Hoover, R. Carriles, D. Kleinfeld, and J. Squier, “Advancing multifocal nonlinear microscopy: development and application of a novel multibeam Yb:KGd(WO4)2 oscillator,” Opt. Express 16, 17574-17584 (2008). [CrossRef] [PubMed]
  18. A. Major, R. Cisek, and V. Barzda, “Femtosecond Yb:KGd(WO4)2 laser oscillator pumped by a high power fiber-coupled diode laser module,” Opt. Express 14, 12163-12168 (2006). [CrossRef] [PubMed]
  19. B. Canfield, H. Husu, J. Kontio, J. Viheriälä, T. Rytkönen, T. Niemi, E. Chandler, A. Hrin, J. Squier, and M. Kauranen, “Inhomogeneities in the nonlinear tensorial responses of arrays of gold nanodots,” New J. Phys. 10, 013001 (2008). [CrossRef]
  20. M. Müller, Introduction to Confocal Fluorescence Microscopy (SPIE, 2006).

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