We report on the design and construction of a confocal light scattering spectroscopic imaging system aimed ultimately to conduct depth-resolved characterization of biological tissues. The confocal sectioning ability of the system is demonstrated using a two-layer sample consisting of a $200\mathrm{\mu m}$ thick cancer cell layer on top of a scattering layer doped with a green absorber. The measurement results demonstrate that distinct light scattering signals can be isolated from each layer with an axial and a lateral resolution of 30 and $27\mathrm{\mu m}$ , respectively. Such a system is expected to have significant applications in the areas of tissue engineering and disease diagnostics and monitoring.

© 2009 Optical Society of America

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Related Journal Articles

• Measurement of Particle Size Distribution in Mammalian Cells In Vitro by Use of Polarized Light Spectroscopy (AO)
• In vivo scattering measurement of biological tissue by the use of a pyroelectric polymer transducer (AO)
• Elastic backscattering spectroscopic microscopy (OL)
• Assessment of blood supply in superficial tissue by polarization-gated elastic light-scattering spectroscopy (AO)
• Method to quantitatively estimate wavelength-dependent scattering properties from multidiameter single fiber reflectance spectra measured in a turbid medium (OL)

Related Conference Papers

• Quantifying the Field Effect of Carcinogenesis with Low-Coherence Enhanced Backscattering Spectroscopy (LEBS)
• An Endoscope Compatible Low-Coherence Enhanced Backscattering Spectroscopy Probe for Cancer Screening
• Minimal scattering events in enhanced backscattering (EBS) of light: Origin of low-coherence EBS in discrete tissue models
• Polarization spectroscopy of early increase in blood supply in predysplastic stages of colon carcinogenesis
• Reconstructing the Low-Coherence Enhanced Backscattering Spectroscopy (LEBS) Peak Using p(r)